Pleural inflammatory response, mesothelin content and DNA damage in mice at one-year after intra-pleural carbon nanotube administration.
Autor: | Wils RS; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5 A, DK-1014 Copenhagen K, Denmark; The National Research Centre for the Working Environment, Lersø Parkalle 105, DK-2100 Copenhagen Ø, Denmark., Jacobsen NR; The National Research Centre for the Working Environment, Lersø Parkalle 105, DK-2100 Copenhagen Ø, Denmark., Vogel U; The National Research Centre for the Working Environment, Lersø Parkalle 105, DK-2100 Copenhagen Ø, Denmark; DTU Food, Technical University of Denmark, DK-2800 Lyngby, Denmark., Roursgaard M; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5 A, DK-1014 Copenhagen K, Denmark., Jensen A; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5 A, DK-1014 Copenhagen K, Denmark., Møller P; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5 A, DK-1014 Copenhagen K, Denmark. Electronic address: pemo@sund.ku.dk. |
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Jazyk: | angličtina |
Zdroj: | Toxicology [Toxicology] 2023 Nov; Vol. 499, pp. 153662. Date of Electronic Publication: 2023 Nov 03. |
DOI: | 10.1016/j.tox.2023.153662 |
Abstrakt: | Many in vitro and in vivo studies have shown that exposure to carbon nanotubes (CNTs) is associated with inflammation, oxidative stress and genotoxicity, although there is a paucity of studies on these effects in the pleural cavity. In the present study, we investigated adverse outcomes of pleural exposure to multi-walled CNTs (MWCNT-7, NM-401 and NM-403) and single-walled CNTs (NM-411). Female C57BL/6 mice were exposed to 0.2 or 5 µg of CNTs by intra-pleural injection and sacrificed one-year post-exposure. Exposure to long and straight types of MWCNTs (i.e. MWCNT-7 and NM-401) was associated with decreased number of macrophages and increased number of neutrophils and eosinophils in pleural lavage fluid. Increased protein content in the pleural lavage fluid was also observed in mice exposed to MWCNT-7 and NM-401. The concentration of mesothelin was increased in mice exposed to MWCNT-7 and NM-411. Levels of DNA strand breaks and DNA oxidation damage, measured by the comet assay, were unaltered in cells from pleural scrape. Extra-pleural effects were seen in CNT exposed mice, including enlarged and pigmented mediastinal lymph nodes (all four types of CNTs), pericardial plaques (MWCNT-7 and NM-401), macroscopic abnormalities on the liver (MWCNT-7) and ovaries/uterus (NM-411). In conclusion, the results demonstrate that intra-pleural exposure to long and straight MWCNTs is associated with adverse outcomes. Certain observations such as increased content of mesothelin in pleural lavage fluid and ovarian/uterine abnormalities in mice exposed to NM-411 suggests that exposure to SWCNTs may also be associated with some adverse outcomes. Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Peter Moller reports financial support was provided by Independent Research Fund Denmark. Ulla Vogel reports financial support was provided by Danish Government. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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