Cytoglobin functions as a redox regulator of melanogenesis in normal epidermal melanocytes.

Autor: Tanaka Y; Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan., Sato-Matsubara M; Department of Hepatology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.; Donated Synthetic Biology Laboratory, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan., Tsuruta D; Department of Dermatology, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan., Tanaka H; Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan., Kadono C; Donated Synthetic Biology Laboratory, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan., Sugawara K; Department of Dermatology, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan., Kawada N; Department of Hepatology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan., Wakamatsu K; Institute for Melanin Chemistry, Fujita Health University, Toyoake, Japan., Ito S; Institute for Melanin Chemistry, Fujita Health University, Toyoake, Japan., Yoshizato K; Donated Synthetic Biology Laboratory, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan.
Jazyk: angličtina
Zdroj: Pigment cell & melanoma research [Pigment Cell Melanoma Res] 2024 Mar; Vol. 37 (2), pp. 276-285. Date of Electronic Publication: 2023 Nov 02.
DOI: 10.1111/pcmr.13146
Abstrakt: Epidermal melanocytes are continuously exposed to sunlight-induced reactive oxygen species (ROS) and oxidative stress generated during the synthesis of melanin. Therefore, they have developed mechanisms that maintain normal redox homeostasis. Cytoglobin (CYGB), a ubiquitously expressed intracellular iron hexacoordinated globin, exhibits antioxidant activity and regulates the redox state of mammalian cells through its activities as peroxidase and nitric oxide (NO) dioxygenase. We postulated that CYGB functions in the melanogenic process as a regulator that maintains oxidative stress within a physiological level. This was examined by characterizing normal human melanocytes with the knockdown (KD) of CYGB using morphological and molecular biological criteria. CYGB-KD cells were larger, had more dendrites, and generated more melanin granules in the advanced stages of melanogenesis than control cells. The expression levels of major melanogenesis-associated genes and proteins were higher in CYGB-KD melanocytes than in wild type (WT) cells. As expected, CYGB-KD melanocytes generated more ROS and NO than WT cells. In conclusion, CYGB physiologically contributes to maintaining redox homeostasis in the melanogenic activity of normal melanocytes by controlling the intracellular levels of ROS and NO.
(© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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