FGF2 Functions in H 2 S's Attenuating Effect on Brain Injury Induced by Deep Hypothermic Circulatory Arrest in Rats.

Autor: Zhu YX; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 61 No. 3 Ave, Binhai District, Tianjin, 300457, People's Republic of China., Yang Q; Center for Basic Medical Research, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Binhai District, Tianjin, 300457, People's Republic of China., Zhang YP; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 61 No. 3 Ave, Binhai District, Tianjin, 300457, People's Republic of China., Liu ZG; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 61 No. 3 Ave, Binhai District, Tianjin, 300457, People's Republic of China. liuzgteda6@163.com.
Jazyk: angličtina
Zdroj: Molecular biotechnology [Mol Biotechnol] 2024 Dec; Vol. 66 (12), pp. 3526-3537. Date of Electronic Publication: 2023 Nov 02.
DOI: 10.1007/s12033-023-00952-3
Abstrakt: Deep hypothermic circulatory arrest (DHCA) can protect the brain during cardiac and aortic surgery by cooling the body, but meanwhile, temporary or permanent brain injury may arise. H 2 S protects neurons and the central nervous system, especially from secondary neuronal injury. We aim to unveil part of the mechanism of H 2 S's attenuating effect on brain injury induced by DHCA by exploring crucial target genes, and further promote the clinical application of H 2 S in DHCA. Nine SD rats were utilized to provide histological and microarray samples, and further the differential expression analysis. Then we conducted GO and KEGG pathway enrichment analyses on candidate genes. The protein-protein interaction (PPI) networks were performed by STRING and GeneMANIA. Crucial target genes' expression was validated by qRT-PCR and western blot. Histological study proved DHCA's damaging effect and H 2 S's repairing effect on brain. Next, we got 477 candidate genes by analyzing differentially expressed genes. The candidate genes were enriched in 303 GO terms and 28 KEGG pathways. Then nine genes were selected as crucial target genes. The function prediction by GeneMANIA suggested their close relation to immunity. FGF2 was identified as the crucial gene. FGF2 plays a vital role in the pathway when H 2 S attenuates brain injury after DHCA. Our research provides more information for understanding the mechanism of H 2 S attenuating brain injury after DHCA. We infer the process might probably be closely associated with immunity.
Competing Interests: Declarations Competing Interests The authors declare that they have no competing interests. Ethical Approval and Consent to Participate The research was approved by the Institutional Ethics Review Board of TEDA International Cardiovascular Hospital (ethic code: TICH-JY-20220715-2). We raised the rats strictly complying with feeding standards and all the experiments were performed in accordance with institutional and national guidelines. Consent for Publication Not applicable.
(© 2023. The Author(s).)
Databáze: MEDLINE
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