Advances in genomic characterization of Urochloa humidicola: exploring polyploid inheritance and apomixis.

Autor: da Costa Lima Moraes A; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Mollinari M; Department of Horticultural Science, Bioinformatics Research Center, North Carolina State University, Raleigh, NC, USA., Ferreira RCU; Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Aono A; Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., de Castro Lara LA; Department of Genetics, University of São Paulo/ESALQ, Piracicaba, São Paulo, Brazil., Pessoa-Filho M; Embrapa Cerrados, Brasília, Distrito Federal, Brazil., Barrios SCL; Embrapa Gado de Corte, Campo Grande, Mato Grosso, Brazil., Garcia AAF; Department of Genetics, University of São Paulo/ESALQ, Piracicaba, São Paulo, Brazil., do Valle CB; Embrapa Gado de Corte, Campo Grande, Mato Grosso, Brazil., de Souza AP; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.; Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Vigna BBZ; Embrapa Pecuária Sudeste, São Carlos, São Paulo, Brazil. bianca.vigna@embrapa.br.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2023 Nov 02; Vol. 136 (11), pp. 238. Date of Electronic Publication: 2023 Nov 02.
DOI: 10.1007/s00122-023-04485-w
Abstrakt: Key Message: We present the highest-density genetic map for the hexaploid Urochloa humidicola. SNP markers expose genetic organization, reproduction, and species origin, aiding polyploid and tropical forage research. Tropical forage grasses are an important food source for animal feeding, with Urochloa humidicola, also known as Koronivia grass, being one of the main pasture grasses for poorly drained soils in the tropics. However, genetic and genomic resources for this species are lacking due to its genomic complexity, including high heterozygosity, evidence of segmental allopolyploidy, and reproduction by apomixis. These complexities hinder the application of marker-assisted selection (MAS) in breeding programs. Here, we developed the highest-density linkage map currently available for the hexaploid tropical forage grass U. humidicola. This map was constructed using a biparental F 1 population generated from a cross between the female parent H031 (CIAT 26146), the only known sexual genotype for the species, and the apomictic male parent H016 (BRS cv. Tupi). The linkage analysis included 4873 single nucleotide polymorphism (SNP) markers with allele dosage information. It allowed mapping of the ASGR locus and apospory phenotype to linkage group 3, in a region syntenic with chromosome 3 of Urochloa ruziziensis and chromosome 1 of Setaria italica. We also identified hexaploid haplotypes for all individuals, assessed the meiotic configuration, and estimated the level of preferential pairing in parents during the meiotic process, which revealed the autopolyploid origin of sexual H031 in contrast to apomictic H016, which presented allopolyploid behavior in preferential pairing analysis. These results provide new information regarding the genetic organization, mode of reproduction, and allopolyploid origin of U. humidicola, potential SNPs markers associated with apomixis for MAS and resources for research on polyploids and tropical forage grasses.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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