Intranasal challenge with B. pertussis leads to more severe disease manifestations in mice than aerosol challenge.

Autor: Weaver KL; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Bitzer GJ; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Wolf MA; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Pyles GM; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., DeJong MA; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Dublin SR; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Huckaby AB; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Gutierrez MP; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Hall JM; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Wong TY; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Warden M; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Petty JE; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Witt WT; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Cunningham C; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Sen-Kilic E; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Damron FH; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America., Barbier M; Vaccine Development Center in the Department of Microbiology, Immunology, and Cell Biology at West Virginia University, Morgantown, WV, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Nov 02; Vol. 18 (11), pp. e0286925. Date of Electronic Publication: 2023 Nov 02 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0286925
Abstrakt: The murine Bordetella pertussis challenge model has been utilized in preclinical research for decades. Currently, inconsistent methodologies are employed by researchers across the globe, making it difficult to compare findings. The objective of this work was to utilize the CD-1 mouse model with two routes of challenge, intranasal and aerosol administration of B. pertussis, to understand the differences in disease manifestation elicited via each route. We observed that both routes of B. pertussis challenge result in dose-dependent colonization of the respiratory tract, but overall, intranasal challenge led to higher bacterial burden in the nasal lavage, trachea, and lung. Furthermore, high dose intranasal challenge results in induction of leukocytosis and pro-inflammatory cytokine responses compared to aerosol challenge. These data highlight crucial differences in B. pertussis challenge routes that should be considered during experimental design.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Weaver et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje