| Autor: |
Young TW; Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, United States., Kappler MP; Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, United States., Hockaden NM; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine-Bloomington, Bloomington, Indiana 47405-7005, United States., Carpenter RL; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine-Bloomington, Bloomington, Indiana 47405-7005, United States., Jacobson SC; Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, United States. |
| Abstrakt: |
Extracellular vesicles (EVs) are cell-derived, naturally produced, membrane-bound nanoscale particles that are linked to cell-cell communication and the propagation of diseases. Here, we report the design and testing of in-plane nanofluidic devices for resistive-pulse measurements of EVs derived from bovine milk and human breast cancer cells. The devices were fabricated in plane with three nanopores in series to determine the particle volume and diameter, two pore-to-pore regions to measure the electrophoretic mobility and zeta potential, and an in-line filter to prevent cellular debris and aggregates from entering the nanopore region. Devices were tested with and without the channels coated with a short-chain PEG silane to minimize electroosmotic flow and permit an accurate measurement of the electrophoretic mobility and zeta potential of the EVs. To enhance throughput of EVs, vacuum was applied to the waste reservoir to increase particle frequencies up to 1000 min -1 . The nanopores had cross-sections 200 nm wide and 200 nm deep and easily resolved EV diameters from 60 to 160 nm. EVs from bovine milk and human breast cancer cells had similar particle size distributions, but their zeta potentials differed by 2-fold, -8 ± 1 and -4 ± 1 mV, respectively. |
