Sex differences and estrogen effects in cardiac mitochondria in human aortic stenosis and in the mouse heart.

Autor:	Fliegner D; Institute of Gender in Medicine, Charité Universitätsmedizin Berlin, Berlin, Germany.; Medical Affairs Internal Medicine, Pfizer Pharma GmbH, Berlin, Germany., Ellieva A; Institute of Gender in Medicine, Charité Universitätsmedizin Berlin, Berlin, Germany., Angelov A; Institute of Gender in Medicine, Charité Universitätsmedizin Berlin, Berlin, Germany., Petrov G; University of Witten- Herdecke, Witten, Germany., Regitz-Zagrosek V; Institute of Gender in Medicine, Charité Universitätsmedizin Berlin, Berlin, Germany.; Clinic for Cardiology, University Hospital Zürich, Zürich, Switzerland.
Jazyk:	angličtina
Zdroj:	Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2023 Oct 17; Vol. 14, pp. 1181044. Date of Electronic Publication: 2023 Oct 17 (Print Publication: 2023).
DOI:	10.3389/fendo.2023.1181044
Abstrakt:	Introduction: Sex differences in the adaptation to pressure overload have been described in humans, as well as animal models, and have been related to sex-specific expression of mitochondrial genes. We therefore tested whether sex differences in cardiac mitochondrial respiration exist in humans with aortic stenosis (AS). We also examined whether these potential differences may be at least partially due to sex hormones by testing if mitochondrial respiration is affected by estrogen (17ß-estradiol (E2)). Methods: Consecutive patients undergoing transapical aortic valve implantation (TAVI) (women, n = 7; men, n = 10) were included. Cardiac biopsies were obtained during TAVI and used directly for mitochondrial function measurements. Male and female C57BL/6J mice (n = 8/group) underwent sham surgery or gonadectomy (GDX) at the age of 2 months. After 14 days, mice were treated once with intraperitoneally injected vehicle (placebo), 17ß-estradiol (E2), estrogen receptor alpha (ERα) agonist [propyl pyrazole triol (PPT)], or ER beta (ERβ) agonist (BAY-1214257). Thereafter, mitochondrial measurements were performed directly in cardiac skinned fibers from isolated left ventricles and musculus solei. Results: Mitochondrial State-3 respiration was higher in female than that in male human heart biopsies (15.0 ± 2.30 vs. 10.3 ± 2.05 nmol/mL/min/mg, p< 0.05). In the mouse model, mitochondrial State-3 respiration decreased significantly after GDX in female (27.6 ± 1.55 vs. 21.4 ± 1.71 nmol/mL/min/mg; p< 0.05) and male hearts (30.7 ± 1,48 vs. 23.7 ± 2,23 nmol/mL/min/mg; p< 0.05). In ovariectomized female mice, E2 and ERβ-agonist treatment restored the State-3 respiration to intact placebo level, whereas ERα-agonist treatment did not modulate State-3 respiration. The treatment with E2, ERα-, or ERβ-agonist did not modulate the State-3 respiration in GDX male mice. Conclusion: We identified sex differences in mitochondrial respiration in the diseased human heart. This is in alignment with known sex differences in the gene expression and proteome level at the functional level. E2 and ERβ affect cardiac mitochondrial function in the mouse model, suggesting that they may also contribute to the sex differences in the human heart. Their roles should be further investigated. Competing Interests: Author DF is employed by the company Pfizer Pharma GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Fliegner, Ellieva, Angelov, Petrov and Regitz-Zagrosek.)
Databáze:	MEDLINE
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