Fullerene C 60 protects against 7,12-dimethylbenz [a] anthracene (DMBA) induced-pancreatic damage via NF-κB and Nrf-2/HO-1 axis in rats.

Autor: Beyaz S; Department of Biology-Molecular Biology and Genetics, Faculty of Science, Firat University, Elazig, Turkey., Aslan A; Department of Biology-Molecular Biology and Genetics, Faculty of Science, Firat University, Elazig, Turkey., Gok O; Department of Biology-Molecular Biology and Genetics, Faculty of Science, Firat University, Elazig, Turkey., Ozercan IH; Department of Pathology, Firat University, Medical School, Elazig, Turkey., Agca CA; Department of Molecular Biology and Genetics Bingol, Faculty of Science, Bingol University, Bingol, Turkey.
Jazyk: angličtina
Zdroj: Toxicology research [Toxicol Res (Camb)] 2023 Sep 26; Vol. 12 (5), pp. 954-963. Date of Electronic Publication: 2023 Sep 26 (Print Publication: 2023).
DOI: 10.1093/toxres/tfad092
Abstrakt: The objective of this investigation was to investigate the protective effects of fullerene C 60 nanoparticle against pancreatic damage experimentally induced by 7,12-dimethylbenz [a] anthracene (DMBA) in female rats. Fullerene C 60 nanoparticle was administered to rats 5 times a week by oral gavage (o.g) at 1.7 mg/kg bw 7 days after DMBA administration. 60 Wistar albino female rats divided to four groups; Groups: (1) Control group: Fed with standard diet; (2) Fullerene C 60 group: Fullerene C 60 (1.7 mg/kg bw); (3) DMBA group: DMBA (45 mg/kg bw); (4) Fullerene C 60  + DMBA group: Fullerene C 60 (1.7 mg/kg bw) and DMBA (45 mg/kg bw). Lipid peroxidation malondialdehyde (MDA), catalase activity (CAT) and glutathione (GSH) levels in pancreatic tissue were determined by spectrophotometer. Protein expression levels of p53, HO-1, p38-α (MAPK), Nrf-2, NF-κB and COX-2 in pancreatic tissue were determined by western blotting technique. In our findings, compared to the group given DMBA, MDA levels and p38-α, NF-κB and COX-2 levels decreased, CAT activity, GSH level, total protein density and p53, HO-1, Nrf-2 levels in the groups given fullerene C 60 nanoparticle an increase in expression levels was observed. Our results showed that fullerene C 60 nanoparticle may be more beneficial in preventing pancreatic damage.
Competing Interests: No potential conflict of interest was reported by the author(s). All authors have read and approved the final article.
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Databáze: MEDLINE
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