Serum butyrylcholinesterase activity in healthy dogs with and without exposure to diazinon.

Autor: Almeida SSF; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., da Silva RHS; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Oliveira IM; Department of Veterinary Clinic and Surgery, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Brazil., Alonso LB; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Gonzaga BS; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Barros ACM; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Vicente MC; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Melo MM; Department of Veterinary Clinic and Surgery, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Brazil., Borges NC; Radiology and Ultrasonography, Clinic and Surgery Sector, Veterinary Hospital, Department of Veterinary Medicine. School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Martins DB; Laboratory of Veterinary Clinical Pathology, Clinic and Surgery Sector, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil., Botelho AFM; Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science, Goiás Federal University, Goiás, Brazil.
Jazyk: angličtina
Zdroj: Veterinary clinical pathology [Vet Clin Pathol] 2023 Dec; Vol. 52 (4), pp. 646-653. Date of Electronic Publication: 2023 Nov 01.
DOI: 10.1111/vcp.13298
Abstrakt: Background: Cholinesterase is a biomarker for poisonings by anticholinesterase agents, but its reference values are scarce, and possible interaction with collars containing parasiticides has not been studied.
Objectives: We aimed to evaluate the serum cholinesterase activity of healthy dogs without a history of contact with anticholinesterase agents and healthy animals exposed to commercial collars containing organophosphate.
Methods: Ninety-nine dogs were used and included healthy animals without recent exposure to anticholinesterase agents and healthy animals previously exposed to diazinon collars. Serum quantification of the enzyme butyrylcholinesterase (BuchE) through spectrophotometry was conducted on all samples. In experiment 1, BuchE activity was quantified at time 0 and 7 days after, a time when the samples were kept at -18°C. In experiment 2, sampling times were 0, 7, 14, 21, 28, and 56 days.
Results: Time 0 values were 4622.38 ± 1311.53 U/L. After 7 days, a significant decay was observed, with a mean of 3934.45 ± 1430.45 U/L. Spearman's test was performed, finding a weak correlation between ALT, creatinine, total plasma proteins, age, weight, red blood cells, platelets, leukocytes, and BuchE activities. In experiment 2, the mean at time 0 was 4753 ± 454.8 U/L. With exposure to the collar, there was a decay of up to 93% after 14 days.
Conclusions: Normality values of serum BuchE in healthy dogs without a history of exposure to anticholinesterase agents were 4360.8-4883.96 U/L. Freezing serum caused a decrease in BuchE activity. Exposure to commercial collars containing diazinon also reduced BuchE activity without clinical signs, indicating that previously exposed animals should be evaluated carefully.
(© 2023 American Society for Veterinary Clinical Pathology.)
Databáze: MEDLINE
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