Reduced expression of miR-221 is associated with the pro-apoptotic pathways in spermatozoa of oligospermia men.

Autor: Saadh MJ; Faculty of Pharmacy, Middle East University, Amman 11831, Jordan; Applied Science Research Center, Applied Science Private University, Amman, Jordan., Pecho RDC; Department of Biochemistry, Universidad San Ignacio de Loyola (USIL), Lima, Peru., Jamal A; Health and Basic Science Research Centre, Majmaah University, Majmaah 11952, Saudi Arabia; Department of Biology, College of Science, Al-Zulfi-, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia., Alothaim AS; Department of Biology, College of Science, Al-Zulfi-, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia., Kamal MA; Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi Arabia., Warsi MK; Department of Biochemistry, College of Science, University of Jeddah, Jeddah 23890, Saudi Arabia., Ahmad F; College of Applied Sciences, Almaarefa University, Diriya, Riyadh 13713, Saudi Arabia., Obaid M; Pharmacy College, Al-Farahidi University, Iraq., Moslem H; Department of Dental Industry Techniques, Al-Noor University College, Nineveh, Iraq., Zainab HA; Department of Pharmacy, Al-Zahrawi University College, Karbala, Iraq., Amin AH; Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt., Arias-Gonzáles JL; Department of Social Sciences, Faculty of Social Studies, University of British Columbia, Arequipa, Peru., Margiana R; Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Master's Programme Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Andrology Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Electronic address: ria.margiana@ui.ac.id., Akhavan-Sigari R; Department of Neurosurgery, University Medical Center Tuebingen, Germany; Department of Health Care Management and Clinical Research, Collegium Humanum Warsaw Management University Warsaw, Poland.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2023 Dec; Vol. 160, pp. 104159. Date of Electronic Publication: 2023 Oct 03.
DOI: 10.1016/j.jri.2023.104159
Abstrakt: Oligospermia and asthenozoospermia, both frequent, can lead to male infertility. Oligospermia might be viewed as a milder form of azoospermia because the same mutations that produce azoospermia in some individuals also create oligospermia in other individuals. In this, we looked at different characteristics of oligospermia men, counting the level of apoptosis and a few related apoptotic and oxidative stress components, and compared them to solid controls. In this study, semen samples from healthy fertile men (n = 35) and oligospermia (n = 35) were collected, and sperm death rates in both groups were examined using flow cytometry. Also, gene expression of apoptotic and anti-apoptotic markers and miR-221 were investigated (Real-Time PCR). Moreover, for the evaluation of catalase and SOD activity and anti-inflammatory cytokines, including IL-10 and TGF-β, the specific ELISA kits and procedures were applied. As a result, higher gene and protein expression levels of PTEN, P27, and P57 were observed in patients with oligospermia. In contrast, lower mRNA expression of AKT and miR-221 was detected in this group. In addition, IL-10, TGF-β, and catalase activity were suppressed in the oligospermia group compared with healthy men samples. Moreover, the frequency of apoptosis of sperm cells is induced in patients. In conclusion, apoptosis-related markers, PTEN, and the measurement of significant and efficient oxidative stress markers like SOD and catalase in semen plasma could be considered as the critical diagnostic markers for oligospermia. Future studies will be better able to treat oligospermia by showing whether these indicators are rising or falling.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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