Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?

Autor: Resseguier J; Section for Physiology and Cell Biology, Departments of Biosciences and Immunology, University of Oslo, Oslo, Norway., Nguyen-Chi M; LPHI, CNRS, Université de Montpellier, Montpellier, France., Wohlmann J; Electron-Microscopy laboratory, Departments of Biosciences, University of Oslo, Oslo, Norway., Rigaudeau D; INRAE, Université Paris-Saclay, IERP, 78350 Jouy-en-Josas, France., Salinas I; Center for Evolutionary and Theoretical Immunology (CETI), Department of Biology, University of New Mexico, Albuquerque, NM, USA., Oehlers SH; A*STAR Infectious Diseases Labs (A*STAR ID Labs), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, Immunos #05-13, Singapore 138648, Singapore., Wiegertjes GF; Aquaculture and Fisheries Group, Department of Animal Sciences, Wageningen University & Research, Wageningen, Netherlands., Johansen FE; Section for Physiology and Cell Biology, Department of Biosciences, University of Oslo, Oslo, Norway., Qiao SW; Department of Immunology, Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Koppang EO; Unit of Anatomy, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway., Verrier B; Laboratory of Tissue Biology and Therapeutic Engineering, UMR 5305, IBCP, CNRS, University Lyon 1, Lyon, France., Boudinot P; Université Paris-Saclay, INRAE, UVSQ, Virologie et Immunologie Moléculaires, Jouy-en-Josas, France., Griffiths G; Section for Physiology and Cell Biology, Department of Biosciences, University of Oslo, Oslo, Norway.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2023 Nov 03; Vol. 9 (44), pp. eadj0101. Date of Electronic Publication: 2023 Nov 01.
DOI: 10.1126/sciadv.adj0101
Abstrakt: The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses to be extremely important for their survival. Here, we used a marker for T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques to investigate the lymphoid architecture of the zebrafish branchial cavity. We identified a sub-pharyngeal lymphoid organ, which we tentatively named "Nemausean lymphoid organ" (NELO). NELO is enriched in T/NK cells, plasma/B cells, and antigen-presenting cells embedded in a network of reticulated epithelial cells. The presence of activated T cells and lymphocyte proliferation, but not V(D)J recombination or hematopoiesis, suggests that NELO is a secondary lymphoid organ. In response to infection, NELO displays structural changes including the formation of T/NK cell clusters. NELO and gill lymphoid tissues form a cohesive unit within a large mucosal lymphoid network. Collectively, we reveal an unreported mucosal lymphoid organ reminiscent of mammalian tonsils that evolved in multiple teleost fish families.
Databáze: MEDLINE
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