Receptor interacting protein kinase-3 mediates both myopathy and cardiomyopathy in preclinical animal models of Duchenne muscular dystrophy.

Autor: Bencze M; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Periou B; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Punzón I; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Barthélémy I; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Taglietti V; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Hou C; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Zaidan L; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Kefi K; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Blot S; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Agbulut O; Institut de Biologie Paris-Seine (IBPS), CNRS UMR 8256, Inserm ERL U1164, Biological Adaptation and Ageing, Sorbonne Université, Paris, France., Gervais M; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Derumeaux G; Team Derumeaux, Department of Physiology, Henri Mondor Hospital, FHU-SENEC, AP-HP, U955-IMRB, Université Paris-Est Créteil (UPEC), Créteil, France., Authier FJ; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Tiret L; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France., Relaix F; Team Relaix, Biology of the Neuromuscular System, U955-IMRB, Inserm, UPEC, ENVA, EFS, Créteil, France.
Jazyk: angličtina
Zdroj: Journal of cachexia, sarcopenia and muscle [J Cachexia Sarcopenia Muscle] 2023 Dec; Vol. 14 (6), pp. 2520-2531. Date of Electronic Publication: 2023 Nov 01.
DOI: 10.1002/jcsm.13265
Abstrakt: Background: Duchenne muscular dystrophy (DMD) is a progressive muscle degenerative disorder, culminating in a complete loss of ambulation, hypertrophic cardiomyopathy and a fatal cardiorespiratory failure. Necroptosis is the form of necrosis that is dependent upon the receptor-interacting protein kinase (RIPK) 3; it is involved in several inflammatory and neurodegenerative conditions. We previously identified RIPK3 as a key player in the acute myonecrosis affecting the hindlimb muscles of the mdx dystrophic mouse model. Whether necroptosis also mediates respiratory and heart disorders in DMD is currently unknown.
Methods: Evidence of activation of the necroptotic axis was examined in dystrophic tissues from Golden retriever muscular dystrophy (GRMD) dogs and R-DMDdel52 rats. A functional assessment of the involvement of necroptosis in dystrophic animals was performed on mdx mice that were genetically depleted for RIPK3. Dystrophic mice aged from 12 to 18 months were analysed by histology and molecular biology to compare the phenotype of muscles from mdxRipk3 +/+ and mdxRipk3 -/- mice. Heart function was also examined by echocardiography in 40-week-old mice.
Results: RIPK3 expression in sartorius and biceps femoris muscles from GRMD dogs positively correlated to myonecrosis levels (r = 0.81; P = 0.0076). RIPK3 was also found elevated in the diaphragm (P ≤ 0.05). In the slow-progressing heart phenotype of GRMD dogs, the phosphorylated form of RIPK1 at the Serine 161 site was dramatically increased in cardiomyocytes. A similar p-RIPK1 upregulation characterized the cardiomyocytes of the severe DMDdel52 rat model, associated with a marked overexpression of Ripk1 (P = 0.007) and Ripk3 (P = 0.008), indicating primed activation of the necroptotic pathway in the dystrophic heart. MdxRipk3 -/- mice displayed decreased compensatory hypertrophy of the heart (P = 0.014), and echocardiography showed a 19% increase in the relative wall thickness (P < 0.05) and 29% reduction in the left ventricle mass (P = 0.0144). Besides, mdxRipk3 -/- mice presented no evidence of a regenerative default or sarcopenia in skeletal muscles, moreover around 50% less affected by fibrosis (P < 0.05).
Conclusions: Our data highlight molecular and histological evidence that the necroptotic pathway is activated in degenerative tissues from dystrophic animal models, including the diaphragm and the heart. We also provide the genetic proof of concept that selective inhibition of necroptosis in dystrophic condition improves both histological features of muscles and cardiac function, suggesting that prevention of necroptosis is susceptible to providing multiorgan beneficial effects for DMD.
(© 2023 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders.)
Databáze: MEDLINE
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