Vulvo-vaginal epithelial tumors in mares: A preliminary investigation on epithelial-mesenchymal transition and tumor-immune microenvironment.

Autor:	Armando F; University of Veterinary Medicine Hannover, Hannover, Germany., Porcellato I; University of Perugia, Perugia, Italy., de Paolis L; University of Perugia, Perugia, Italy.; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Genova, Italy., Mecocci S; University of Perugia, Perugia, Italy., Passeri B; University of Parma, Parma, Italy., Ciurkiewicz M; University of Veterinary Medicine Hannover, Hannover, Germany., Mechelli L; University of Perugia, Perugia, Italy., Grazia De Ciucis C; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Genova, Italy.; University of Pavia, Pavia, Italy., Pezzolato M; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Genova, Italy., Fruscione F; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Genova, Italy., Brachelente C; University of Perugia, Perugia, Italy., Montemurro V; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Genova, Italy., Cappelli K; University of Perugia, Perugia, Italy., Puff C; University of Veterinary Medicine Hannover, Hannover, Germany., Baumgärtner W; University of Veterinary Medicine Hannover, Hannover, Germany., Ghelardi A; Ospedale Apuane, Massa, Italy., Razzuoli E; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Genova, Italy.
Jazyk:	angličtina
Zdroj:	Veterinary pathology [Vet Pathol] 2024 May; Vol. 61 (3), pp. 366-381. Date of Electronic Publication: 2023 Nov 01.
DOI:	10.1177/03009858231207025
Abstrakt:	Vulvo-vaginal epithelial tumors are uncommon in mares, and data on the epithelial-to-mesenchymal transition (EMT) and the tumor-immune microenvironment (TIME) are still lacking. This is a study investigating the equus caballus papillomavirus type 2 (EcPV2) infection state as well as the EMT process and the tumor microenvironment in vulvo-vaginal preneoplastic/ benign (8/22) or malignant (14/22) epithelial lesions in mares. To do this, histopathological, immunohistochemical, transcriptomic, in situ hybridization, and correlation analyses were carried out. Immunohistochemistry quantification showed that cytoplasmic E-cadherin and β-catenin expression as well as nuclear β-catenin expression were features of malignant lesions, while benign/preneoplastic lesions were mainly characterized by membranous E-cadherin and β-catenin expression. Despite this, there were no differences between benign and malignant equine vulvo-vaginal lesions in the expression of downstream genes involved in the canonical and noncanonical wnt/β-catenin pathways. In addition, malignant lesions were characterized by a lower number of cells with cytoplasmic cytokeratin expression as well as a slightly higher cytoplasmic vimentin immunolabeling. The TIME of malignant lesions was characterized by more numerous CD204 + M2-polarized macrophages. Altogether, our results support the hypothesis that some actors in TIME such as CD204 + M2-polarized macrophages may favor the EMT process in equine vulvo-vaginal malignant lesions providing new insights for future investigations in the field of equine EcPV2-induced genital neoplastic lesions. Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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