The association of Treg and Th17 cells development factors and anti-TPO autoantibodies in patients with recurrent pregnancy loss.

Autor: Niafar M; Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Samaie V; Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Soltani-Zangbar MS; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Immunology, Faculty of medicine, Tabriz University of Medical Sciences, Tabriz, Iran., Motavalli R; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Molecular Medicine, Faculty of Advanced Medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran., Dolati S; Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran., Danaii S; Gynecology Departments, ACECR ART Center, Eastern Azerbaijan Branch of ACECR, Eastern, Tabriz, Iran., Mehdizadeh A; Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Yousefi M; Department of Immunology, Faculty of medicine, Tabriz University of Medical Sciences, Tabriz, Iran. mehdi_yusefi@yahoo.com.; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. mehdi_yusefi@yahoo.com.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2023 Oct 31; Vol. 16 (1), pp. 302. Date of Electronic Publication: 2023 Oct 31.
DOI: 10.1186/s13104-023-06579-6
Abstrakt: Objectives: Thyroid autoimmunity is considered as the most prevalent autoimmune condition in women in fertility age. There are different clinical evidences indicating the association between thyroid autoimmunity and increased risk of RPL. This study aimed to analyze the association of Tregs and Th17 cells development factors and anti-thyroid peroxidase (anti-TPO) antibodies in RPL patients. Healthy controls (n = 36), TPO + controls (n = 25) and TPO + RPL (n = 32) participated in this study. After blood sampling, the frequency of Th17 and Tregs was evaluated using flow cytometry. Real-time PCR and ELISA was used to assess the status of Tregs and Th17 related transcription factors and cytokines in mRNA and protein level, respectively.
Results: TPO + RPL group showed a higher Th17 frequency compared to healthy controls and TPO + controls groups (p = 0.0002 and p = 0.04, respectively). Additionally, mRNA expression levels of RORγT and IL-17 were significantly higher in TPO + RPL compared to healthy controls and TPO + controls groups. In contrast, Foxp3 and TGFβ expression was lower in TPO + RPL. ELISA findings also indicated a significantly higher IL-17 and lower TGFβ secretion in TPO + RPL compared to healthy controls and TPO + controls. Thyroid autoimmunity should intensely be controlled specially in patients with RPL history.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje