Ascorbic Acid 2-Phosphate Releasing Supercritically Foamed Porous Poly-L-Lactide-Co-ε-Caprolactone Scaffold Enhances the Collagen Production of Human Vaginal Stromal Cells: A New Approach for Vaginal Tissue Engineering.

Autor: Sartoneva R; Faculty of Medicine and Health Technology (MET), Tampere University, Arvo Ylpön Katu 34, 33520, Tampere, Finland. reetta.sartoneva@tuni.fi.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Arvo Ylpön Katu 34, 33520, Tampere, Finland. reetta.sartoneva@tuni.fi.; Department of Obstetrics and Gynaecology, Seinäjoki Central Hospital, Seinäjoki, Finland. reetta.sartoneva@tuni.fi., Paakinaho K; Faculty of Medicine and Health Technology (MET), Tampere University, Arvo Ylpön Katu 34, 33520, Tampere, Finland.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Arvo Ylpön Katu 34, 33520, Tampere, Finland., Hannula M; Faculty of Medicine and Health Technology (MET), Tampere University, Arvo Ylpön Katu 34, 33520, Tampere, Finland.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Arvo Ylpön Katu 34, 33520, Tampere, Finland., Kuismanen K; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Arvo Ylpön Katu 34, 33520, Tampere, Finland.; Department of Obstetrics and Gynaecology, Tampere University Hospital, Tampere, Finland., Huhtala H; Faculty of Social Sciences, University of Tampere, Tampere, Finland., Hyttinen J; Faculty of Medicine and Health Technology (MET), Tampere University, Arvo Ylpön Katu 34, 33520, Tampere, Finland.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Arvo Ylpön Katu 34, 33520, Tampere, Finland., Miettinen S; Faculty of Medicine and Health Technology (MET), Tampere University, Arvo Ylpön Katu 34, 33520, Tampere, Finland.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Arvo Ylpön Katu 34, 33520, Tampere, Finland.
Jazyk: angličtina
Zdroj: Tissue engineering and regenerative medicine [Tissue Eng Regen Med] 2024 Jan; Vol. 21 (1), pp. 81-96. Date of Electronic Publication: 2023 Oct 31.
DOI: 10.1007/s13770-023-00603-3
Abstrakt: Background: The reconstructive surgery of vaginal defects is highly demanding and susceptible to complications, especially in larger defects requiring nonvaginal tissue grafts. Thus, tissue engineering-based solutions could provide a potential approach to the reconstruction of vaginal defects.
Methods: Here, we evaluated a novel porous ascorbic acid 2-phosphate (A2P)-releasing supercritical carbon dioxide foamed poly-L-lactide-co-ε-caprolactone (scPLCL A2P ) scaffold for vaginal reconstruction with vaginal epithelial (EC) and stromal (SC) cells. The viability, proliferation, and phenotype of ECs and SCs were evaluated in monocultures and in cocultures on d 1, d 7 and d 14. Furthermore, the collagen production of SCs on scPLCL A2P was compared to that on scPLCL without A2P on d 14.
Results: Both ECs and SCs maintained their viability on the scPLCL A2P scaffold in mono- and coculture conditions, and the cells maintained their typical morphology during the 14-d culture period. Most importantly, the scPLCL A2P scaffolds supported the collagen production of SCs superior to plain scPLCL based on total collagen amount, collagen I and III gene expression results and collagen immunostaining results.
Conclusion: This is the first study evaluating the effect of A2P on vaginal tissue engineering, and the results are highly encouraging, indicating that scPLCL A2P has potential as a scaffold for vaginal tissue engineering.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: