Microaerobic methane-driven denitrification in a biotrickle bed - Investigating the active microbial biofilm community composition using RNA-stable isotope probing.

Autor: Egbadon EO; Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand., Wigley K; Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand., Nwoba ST; Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand., Carere CR; Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand., Weaver L; Institute of Environmental Science and Research Ltd., Christchurch, New Zealand., Baronian K; Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand., Burbery L; Institute of Environmental Science and Research Ltd., Christchurch, New Zealand., Gostomski PA; Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand. Electronic address: peter.gostomski@canterbury.ac.nz.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Jan; Vol. 346, pp. 140528. Date of Electronic Publication: 2023 Oct 30.
DOI: 10.1016/j.chemosphere.2023.140528
Abstrakt: A microaerobic (2% O 2 v/v) biotrickle bed reactor supplied continuously with 2% methane to drive nitrate removal (MAME-D) was investigated using 16S rDNA and rRNA amplicon sequencing in combination with RNA-stable isotope probing (RNA-SIP) to identify the active microorganisms. Methane removal rates varied from 500 to 1000 mmol m -3 h -1 and nitrate removal rates from 25 to 58 mmol m -3 h -1 over 55 days of operation. Biofilm samples from the column were incubated in serum bottles supplemented with 13 CH 4 . 16S rDNA analysis indicated a simple community structure in which four taxa accounted for 45% of the total relative abundance (RA). Dominant genera included the methanotroph Methylosinus and known denitrifiers Nubsella and Pseudoxanthomonas; along with a probable denitrifier assigned to the order Obscuribacterales. The 16S rRNA results revealed the methanotrophs Methylocystis (15% RA) and Methylosinus (10% RA) and the denitrifiers Arenimonas (10% RA) and Pseudoxanthomonas (7% RA) were the most active genera. Obscuribacterales was the most active taxa in the community at 22% RA. Activity was confirmed by the Δ buoyant density changes with time for the taxa, indicating most of the community activity was associated with methane oxidation and subsequent consumption of methanotrophic metabolic intermediates by the denitrifiers. This is the first report of RNA stable isotope probing within a microaerobic methane driven denitrification system and the active community was markedly different from the full community identified via 16S-rDNA analysis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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