Ethanol suppresses rice seed germination through inhibiting ROS signaling.

Autor: Chen J; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Jin Z; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Xiang L; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Chen Y; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Zhang J; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Zhao J; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Huang F; Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Shi Y; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 310006, PR China., Cheng F; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China., Pan G; Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: pangang12@zju.edu.cn.
Jazyk: angličtina
Zdroj: Journal of plant physiology [J Plant Physiol] 2023 Dec; Vol. 291, pp. 154123. Date of Electronic Publication: 2023 Oct 20.
DOI: 10.1016/j.jplph.2023.154123
Abstrakt: Ethanol is frequently used not only as priming but also as a solvent to dissolve hardly water-soluble phytohormones gibberellic acid (GA 3 ) and abscisic acid (ABA) in seed germination. However, the molecular and physiological mechanisms of ethanol's impact on seed germination remain elusive. In this report, we investigated how ethanol affected reactive oxygen species (ROS) during rice seed germination. Ethanol at a concentration of 3.5% (v/v) inhibited 90% seed germination, which was almost reversed by H 2 O 2 . H 2 O 2 contents in embryos were reduced by ethanol after 18 h imbibition. Antioxidant enzymes assays revealed that only superoxide dismutase (SOD) activities in seed embryos were lowered by ethanol, in line with the suppressed mRNA expression of SOD genes during imbibition. Additionally, compared to the mock condition, ethanol increased ABA contents but decreased GA (GA 1 and GA 3 ) in seed embryos, resulting in disharmonizing GA/ABA balance. Conceivably ethanol induced transcription of OsNCEDs, the key genes for ABA biosynthesis, and OsABA8ox3, a key gene for ABA catabolism. Furthermore, ethanol promoted ABA signaling by upregulating ABA receptor genes and ABA-responsive element (ABRE)-binding protein/ABRE-binding factors during imbibition. Overall, our results demonstrate that lowering of H 2 O 2 levels due to suppressed SOD activities in rice germinating seed embryos is the decisive factor for ethanol-induced inhibition of seed germination, and GA/ABA balance and ABA signaling also play important roles in ethanol's inhibitory impact on seed germination.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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