Interferon-tau (IFN-τ) Has Antiproliferative Effects, Induces Apoptosis, and Inhibits Tumor Growth in a Triple-negative Breast Cancer Murine Tumor Model.

Autor:	Espin-Rivera AM; Division of Chronic Infection and Cancer, Research Center of Infectious Diseases, National Institute of Public Health, Cuernavaca, México., Meza-Aparicio FU; Division of Chronic Infection and Cancer, Research Center of Infectious Diseases, National Institute of Public Health, Cuernavaca, México., Reyna-Flores F; Division of Chronic Infection and Cancer, Research Center of Infectious Diseases, National Institute of Public Health, Cuernavaca, México., Burguete-Garcia AI; Division of Chronic Infection and Cancer, Research Center of Infectious Diseases, National Institute of Public Health, Cuernavaca, México., Guzman-Olea E; Catedratico Consejo Nacional de Ciencia y Tecnología (CONACYT), Institute of Health Sciences, Autonomous University of Hidalgo State, Hidalgo, México., Bermudez-Morales VH; Division of Chronic Infection and Cancer, Research Center of Infectious Diseases, National Institute of Public Health, Cuernavaca, México; vbermudez@insp.mx.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2023 Nov-Dec; Vol. 37 (6), pp. 2517-2523.
DOI:	10.21873/invivo.13359
Abstrakt:	Background/aim: Resistant triple-negative breast cancer (TNBC) is a subtype of this disease that is resistant to conventional chemotherapy agents. IFN-τ is a cytokine that has recently been shown to have immunoregulatory and antitumor effects. The present study aimed to examine the antiproliferative and apoptosis effects of IFN-τ in breast cancer cells and the antitumor effect in a murine tumor model of TNBC. Materials and Methods: Murine breast cancer 4T1 cells were cultured and treated with ovine IFN-τ and through MTT and Caspase-Glo 3/7 assays, viability and cell death were determined. In addition, the antitumor effect of IFN-τ was determined in a murine tumor model of TNBC. Results: Ovine IFN-τ showed a concentration-dependent antiproliferative effect on 4T1 murine breast cancer cells. Also, treatment of 4T1 cells with IFN-τ induced the activation of caspase 3 and 7, which is indicative of apoptotic cell death. Moreover, we detected an increase in the expression of type I interferon receptor (IFNAR1/2) in cells treated with IFN-. The intratumoral application of IFN-τ in mice inhibited tumor growth compared to the control non-treated group, and the effect was associated with the increased expression of GM-CSF. Conclusion: Ovine IFN-τ may be an effective immunotherapeutic cytokine for the treatment of TNBC. (Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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