Effects of squamous cell carcinoma and smoking status on oropharyngeal and laryngeal microbial communities.

Autor: Oberste M; Department of Otorhinolaryngology - Head and Neck Surgery, University Hospital of Münster, Münster, Germany., Böse BE; Department of Otorhinolaryngology - Head and Neck Surgery, University Hospital of Münster, Münster, Germany., Dos Anjos Borges LG; Research Group Microbial Interactions and Processes, Helmholtz Centre for Infection Research, Braunschweig, Germany., Junca H; Research Group Microbial Interactions and Processes, Helmholtz Centre for Infection Research, Braunschweig, Germany., Plumeier I; Research Group Microbial Interactions and Processes, Helmholtz Centre for Infection Research, Braunschweig, Germany., Kahl S; Research Group Microbial Interactions and Processes, Helmholtz Centre for Infection Research, Braunschweig, Germany., Simon F; Department of Otorhinolaryngology - Head and Neck Surgery, University Hospital of Münster, Münster, Germany., Beule AG; Department of Otorhinolaryngology - Head and Neck Surgery, University Hospital of Münster, Münster, Germany., Rudack C; Department of Otorhinolaryngology - Head and Neck Surgery, University Hospital of Münster, Münster, Germany., Pieper DH; Research Group Microbial Interactions and Processes, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Jazyk: angličtina
Zdroj: Head & neck [Head Neck] 2024 Jan; Vol. 46 (1), pp. 145-160. Date of Electronic Publication: 2023 Oct 31.
DOI: 10.1002/hed.27562
Abstrakt: Background: Still, little is known about microbial dysbiosis in oropharyngeal and laryngeal tissue as risk factor for development of local squamous cell carcinoma. The site-specific microbiota at these regions in healthy and cancer tissue and their modulation by environmental factors need to be defined.
Methods: The local microbiota of cancer tissue and healthy controls was profiled by 16S rRNA gene amplicon sequencing and statistical analysis using 111 oropharyngeal and 72 laryngeal intraoperative swabs.
Results: Oropharynx and larynx harbor distinct microbial communities. Clear effects of both smoking and cancer were seen in the oropharynx whereas effects in the larynx were minor.
Conclusion: The distinct microbial communities at larynx and oropharynx partially explain why the effects of cancer and smoking were distinct at those sites. Thus, the use of microbiota supposed to mirror community changes in another target location should be avoided and more studies on the actual cancerous environment are necessary.
(© 2023 The Authors. Head & Neck published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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