Microglia are dispensable for experience-dependent refinement of visual circuitry.

Autor:	Brown TC; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202., Crouse EC; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202., Attaway CA; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202., Oakes DK; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202., Minton SW; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202., Borghuis BG; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202., McGee AW; Department of Anatomical Sciences and Neurobiology, School of Medicine; University of Louisville, Louisville, KY, 40202.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Oct 17. Date of Electronic Publication: 2023 Oct 17.
DOI:	10.1101/2023.10.17.562708
Abstrakt:	Microglia are proposed to be critical for the refinement of developing neural circuitry. However, evidence identifying specific roles for microglia has been limited and often indirect. Here we examined whether microglia are required for the experience-dependent refinement of visual circuitry and visual function during development. We ablated microglia by administering the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622, and then examined the consequences for retinal function, receptive field tuning of neurons in primary visual cortex (V1), visual acuity, and experience-dependent plasticity in visual circuitry. Eradicating microglia by treating mice with PLX5622 beginning at postnatal day (P) 14 did not alter visual response properties of retinal ganglion cells examined three or more weeks later. Mice treated with PLX5622 from P14 lacked more than 95% of microglia in V1 by P18, prior to the opening of the critical period. Despite the absence of microglia, the receptive field tuning properties of neurons in V1 were normal at P32. Similarly, eradicating microglia did not affect the maturation of visual acuity. Mice treated with PLX5622 displayed typical ocular dominance plasticity in response to brief monocular deprivation. Thus, none of these principal measurements of visual circuit development and function detectibly differed in the absence of microglia. We conclude that microglia are dispensable for experience-dependent refinement of visual circuitry. These findings challenge the proposed critical role of microglia in refining neural circuitry. Competing Interests: Competing interests The authors have declared that no competing interests exist.
Databáze:	MEDLINE
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