ROS-producing nanomaterial engineered from Cu(I) complexes with P 2 N 2 -ligands for cancer cells treating.

Autor: Faizullin BA; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088. bulat_fayzullin95@mail.ru., Dayanova IR; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Kurenkov AV; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Gubaidullin AT; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Saifina AF; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Nizameev IR; Department of Physics, Kazan National Research Technological University, 68 Karl Marx Str., Kazan, Russia, 420015., Kholin KV; Department of Nanotechnology in Electronics, Kazan National Research Technical University Named After A.N. Tupolev-KAI, 10 K. Marx Street, Kazan, Russia, 420111., Khrizanforov MN; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088.; Aleksander Butlerov Institute of Chemistry, Kazan Federal University, 1/29 Lobachevski Str., Kazan, Russia, 420008., Sirazieva AR; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Litvinov IA; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Voloshina AD; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Lyubina AP; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Sibgatullina GV; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2/31 Lobachevski Str., Kazan, Russia, 420111., Samigullin DV; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2/31 Lobachevski Str., Kazan, Russia, 420111.; Institute for Radio-Electronics and Telecommunications, Kazan National Research Technical University Named After A.N. Tupolev-KAI, 10 K. Marx Street, Kazan, Russia, 420111., Musina EI; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Strelnik ID; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088.; Aleksander Butlerov Institute of Chemistry, Kazan Federal University, 1/29 Lobachevski Str., Kazan, Russia, 420008., Karasik AA; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088., Mustafina AR; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Str., Kazan, Russia, 420088.
Jazyk: angličtina
Zdroj: Discover nano [Discov Nano] 2023 Oct 30; Vol. 18 (1), pp. 133. Date of Electronic Publication: 2023 Oct 30.
DOI: 10.1186/s11671-023-03912-7
Abstrakt: The work presents core-shell nanoparticles (NPs) built from the novel Cu(I) complexes with cyclic P 2 N 2 -ligands (1,5-diaza-3,7-diphosphacyclooctanes) that can visualize their entry into cancer and normal cells using a luminescent signal and treat cells by self-enhancing generation of reactive oxygen species (ROS). Variation of P- and N-substituents in the series of P 2 N 2 -ligands allows structure optimization of the Cu(I) complexes for the formation of the luminescent NPs with high chemical stability. The non-covalent modification of the NPs with triblock copolymer F-127 provides their high colloidal stability, followed by efficient cell internalization of the NPs visualized by their blue (⁓450 nm) luminescence. The cytotoxic effects of the NPs toward the normal and some of cancer cells are significantly lower than those of the corresponding molecular complexes, which correlates with the chemical stability of the NPs in the solutions. The ability of the NPs to self-enhanced and H 2 O 2 -induced ROS generation is demonstrated in solutions and intracellular space by means of the standard electron spin resonance (ESR) and fluorescence techniques correspondingly. The anticancer specificity of the NPs toward HuTu 80 cancer cells and the apoptotic cell death pathway correlate with the intracellular level of ROS, which agrees well with the self-enhancing ROS generation of the NPs. The enhanced level of ROS revealed in HuTu 80 cells incubated with the NPs can be associated with the significant level of their mitochondrial localization.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: