Light-Activated Assembly of DNA Origami into Dissipative Fibrils.

Autor: Berg WR; School of Chemistry, University of New South Wales, Sydney, 2052, Australia.; Institut für Chemie und Biochemie, Freie Universität Berlin, Fabeckstraße 34-36, 14195, Berlin, Germany., Berengut JF; EMBL Australia Node for Single Molecule Science, School of Biomedical Sciences, University of New South Wales, Sydney, 2052, Australia.; ARC Centre of Excellence in Synthetic Biology, University of New South Wales, Sydney, 2052, Australia., Bai C; School of Chemistry, University of New South Wales, Sydney, 2052, Australia., Wimberger L; School of Chemistry, University of New South Wales, Sydney, 2052, Australia., Lee LK; EMBL Australia Node for Single Molecule Science, School of Biomedical Sciences, University of New South Wales, Sydney, 2052, Australia.; ARC Centre of Excellence in Synthetic Biology, University of New South Wales, Sydney, 2052, Australia., Rizzuto FJ; School of Chemistry, University of New South Wales, Sydney, 2052, Australia.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2023 Dec 18; Vol. 62 (51), pp. e202314458. Date of Electronic Publication: 2023 Nov 14.
DOI: 10.1002/anie.202314458
Abstrakt: Hierarchical DNA nanostructures offer programmable functions at scale, but making these structures dynamic, while keeping individual components intact, is challenging. Here we show that the DNA A-motif-protonated, self-complementary poly(adenine) sequences-can propagate DNA origami into one-dimensional, micron-length fibrils. When coupled to a small molecule pH regulator, visible light can activate the hierarchical assembly of our DNA origami into dissipative fibrils. This system is recyclable and does not require DNA modification. By employing a modular and waste-free strategy to assemble and disassemble hierarchical structures built from DNA origami, we offer a facile and accessible route to developing well-defined, dynamic, and large DNA assemblies with temporal control. As a general tool, we envision that coupling the A-motif to cycles of dissipative protonation will allow the transient construction of diverse DNA nanostructures, finding broad applications in dynamic and non-equilibrium nanotechnology.
(© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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