Multi-omics analyses reveal the importance of chromoplast plastoglobules in carotenoid accumulation in citrus fruit.

Autor: Liu Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China.; Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P.R. China., Ye J; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Zhu M; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Atkinson RG; The New Zealand Institute for Plant and Food Research Ltd (PFR), Private Bag, 92169, Auckland, New Zealand., Zhang Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Zheng X; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Lu J; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Cao Z; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Peng J; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Shi C; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Xie Z; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Larkin RM; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Nieuwenhuizen NJ; The New Zealand Institute for Plant and Food Research Ltd (PFR), Private Bag, 92169, Auckland, New Zealand., Ampomah-Dwamena C; The New Zealand Institute for Plant and Food Research Ltd (PFR), Private Bag, 92169, Auckland, New Zealand., Chen C; Guangxi Academy of Specialty Crops/Guangxi Engineering Research Center of Citrus Breeding and Culture, Guilin, 541004, P.R. China., Wang R; Shanghai Applied Protein Technology Co. Ltd, Shanghai, 200233, P.R. China., Luo X; Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P.R. China., Cheng Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Deng X; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China., Zeng Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, P.R. China.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Feb; Vol. 117 (3), pp. 924-943. Date of Electronic Publication: 2023 Oct 30.
DOI: 10.1111/tpj.16519
Abstrakt: Chromoplasts act as a metabolic sink for carotenoids, in which plastoglobules serve as versatile lipoprotein particles. PGs in chloroplasts have been characterized. However, the features of PGs from non-photosynthetic plastids are poorly understood. We found that the development of chromoplast plastoglobules (CPGs) in globular and crystalloid chromoplasts of citrus is associated with alterations in carotenoid storage. Using Nycodenz density gradient ultracentrifugation, an efficient protocol for isolating highly purified CPGs from sweet orange (Citrus sinensis) pulp was established. Forty-four proteins were defined as likely comprise the core proteome of CPGs using comparative proteomics analysis. Lipidome analysis of different chromoplast microcompartments revealed that the nonpolar microenvironment within CPGs was modified by 35 triacylglycerides, two sitosterol esters, and one stigmasterol ester. Manipulation of the CPG-localized gene CsELT1 (esterase/lipase/thioesterase) in citrus calli resulted in increased lipids and carotenoids, which is further evidence that the nonpolar microenvironment of CPGs contributes to carotenoid accumulation and storage in the chromoplasts. This multi-feature analysis of CPGs sheds new light on the role of chromoplasts in carotenoid metabolism, paving the way for manipulating carotenoid content in citrus fruit and other crops.
(© 2023 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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