Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020.

Autor:	Gin TE; College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States of America., Petzold EA; Department of Infectious Diseases, Duke University, Durham, NC, United States of America., Uthappa DM; Hubert-Yeargan Center for Global Health, Duke University, Durham, NC, United States of America., Neighbors CE; Hubert-Yeargan Center for Global Health, Duke University, Durham, NC, United States of America., Borough AR; Hubert-Yeargan Center for Global Health, Duke University, Durham, NC, United States of America., Gin C; College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States of America., Lashnits E; Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United States of America., Sempowski GD; Duke Human Vaccine Institute, Duke University, Durham, NC, United States of America., Denny T; Duke Human Vaccine Institute, Duke University, Durham, NC, United States of America., Bienzle D; Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada., Weese JS; Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada., Callahan BJ; College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States of America., Woods CW; Department of Infectious Diseases, Duke University, Durham, NC, United States of America.; Hubert-Yeargan Center for Global Health, Duke University, Durham, NC, United States of America.; Duke Human Vaccine Institute, Duke University, Durham, NC, United States of America.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Oct 24; Vol. 11, pp. e16310. Date of Electronic Publication: 2023 Oct 24 (Print Publication: 2023).
DOI:	10.7717/peerj.16310
Abstrakt:	We collected oral and/or rectal swabs and serum from dogs and cats living in homes with SARS-CoV-2-PCR-positive persons for SARS-CoV-2 PCR and serology testing. Pre-COVID-19 serum samples from dogs and cats were used as negative controls, and samples were tested in duplicate at different timepoints. Raw ELISA results scrutinized relative to known negative samples suggested that cut-offs for IgG seropositivity may require adjustment relative to previously proposed values, while proposed cut-offs for IgM require more extensive validation. A small number of pet dogs (2/43, 4.7%) and one cat (1/21, 4.8%) were positive for SARS-CoV-2 RNA, and 28.6 and 37.5% of cats and dogs were positive for anti-SARS-CoV-2 IgG, respectively. Competing Interests: Dorothee Bienzle is an Academic Editor for PeerJ. (©2023 Gin et al.)
Databáze:	MEDLINE
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