Serological and Molecular Epidemiology of Chikungunya Virus Infection in Vietnam, 2017-2019.

Autor: Nguyen TV; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523, Japan.; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Ngwe Tun MM; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.; DEJIMA Infectious Disease Research Alliance, Nagasaki University, Nagasaki 852-8523, Japan.; Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo 690-8504, Japan., Cao MT; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Dao HM; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Luong CQ; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Huynh TKL; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Nguyen TTT; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Hoang TND; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Morita K; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.; DEJIMA Infectious Disease Research Alliance, Nagasaki University, Nagasaki 852-8523, Japan., Le TQM; National Institute of Hygiene and Epidemiology, Hanoi 100000, Vietnam., Pham QD; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Takamatsu Y; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.; DEJIMA Infectious Disease Research Alliance, Nagasaki University, Nagasaki 852-8523, Japan., Hasebe F; Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2023 Oct 08; Vol. 15 (10). Date of Electronic Publication: 2023 Oct 08.
DOI: 10.3390/v15102065
Abstrakt: Chikungunya fever is an acute febrile illness caused by the chikungunya virus (CHIKV), which is transmitted by Aedes mosquitoes. Since 1965, only a few studies with limited scope have been conducted on CHIKV in Vietnam. Thus, this study aimed to determine the seroprevalence and molecular epidemiology of CHIKV infection among febrile patients in Vietnam from 2017 to 2019. A total of 1063 serum samples from 31 provinces were collected and tested for anti-CHIKV IgM and IgG ELISA. The 50% focus reduction neutralization test (FRNT 50 ) was used to confirm CHIKV-neutralizing antibodies. Quantitative real-time RT-PCR (RT-qPCR) was performed to confirm the presence of the CHIKV genome. The results showed that 15.9% (169/1063) of the patients had anti-CHIKV IgM antibodies, 20.1% (214/1063) had anti-CHIKV IgG antibodies, 10.4% (111/1063) had CHIKV-neutralizing antibodies, and 27.7% (130/469) of the samples were positive in RT-qPCR analysis. The E1 CHIKV genome sequences were detected among the positive RT-qPCR samples. Our identified sequences belonged to the East/Central/South/African (ECSA) genotype, which has been prevalent in Vietnam previously, suggesting CHIKV has been maintained and is endemic in Vietnam. This study demonstrates a high prevalence of CHIKV infection in Vietnam and calls for an annual surveillance program to understand its impact.
Databáze: MEDLINE
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