| Autor: |
Huneif MA; Pediatric Department, Medical College, Najran University, Najran 61441, Saudi Arabia., Fahad S; Department of Agronomy, Abdul Wali Khan University Mardan, Mardan 23200, KP, Pakistan., Abdulwahab A; Pediatric Department, Medical College, Najran University, Najran 61441, Saudi Arabia., Alqahtani SM; Pediatric Department, Medical College, Najran University, Najran 61441, Saudi Arabia., Mahnashi MH; Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran 61441, Saudi Arabia., Nawaz A; Department of Pharmacy, Faculty of Biological Sciences, University of Malakand, Chakdara 18000, KP, Pakistan., Hussain F; Department of Pharmacy, University of Swabi, Swabi 23561, KP, Pakistan., Sadiq A; Department of Pharmacy, Faculty of Biological Sciences, University of Malakand, Chakdara 18000, KP, Pakistan. |
| Jazyk: |
angličtina |
| Zdroj: |
Plants (Basel, Switzerland) [Plants (Basel)] 2023 Oct 17; Vol. 12 (20). Date of Electronic Publication: 2023 Oct 17. |
| DOI: |
10.3390/plants12203591 |
| Abstrakt: |
Diabetes mellitus (DM) is a metabolic complication and can pose a serious challenge to human health. DM is the main cause of many life-threatening diseases. Researchers of natural products have been continuously engaged in treating vital diseases in an economical and efficient way. In this research, we extensively used phytosteroids from Notholirion thomsonianum (Royle) Stapf for the treatment of DM. The structures of phytosteroids NtSt01 and NtSt02 were confirmed with gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses. Through in vitro studies including α-glucosidase, α-amylase, and DPPH assays, compound NtSt01 was found to be comparatively potent. An elevated dose of compound NtSt01 was also found to be safe in an experimental study on rats. With a dose of 1.0 mg/kg of NtSt01 , the effect on blood glucose levels in rats was observed to be 519 ± 3.98, 413 ± 1.87, 325 ± 1.62, 219 ± 2.87, and 116 ± 1.33 mg/dL on the 1st, 7th, 14th, 21st, and 28th, days, respectively. The in vivo results were compared with those of glibenclamide, which reduced the blood glucose level to 107 ± 2.33 mg/dL on the 28th day. On the 28th day of NtSt01 administration, the average weights of the rats and vital organs (liver, kidney, pancreas, and heart) remained healthy, with a slight increase. The biochemical parameters of the blood, i.e., serum creatinine, blood urea, serum bilirubin, SGPT (or ALT), and serum alkaline phosphatase, of rats treated with NtSt01 remained in the normal ranges. Similarly, the serum cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels also remained within the standard ranges. It is obvious from our overall results that the phytosteroids (specifically NtSt01 ) had an efficient therapeutic effect on the blood glucose level, protection of vital organs, and blood biochemistry. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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