| Autor: |
Wang J; College of Plant Protection, Northeast Agricultural University, Harbin 150030, China.; College of Agriculture, Northeast Agricultural University, Harbin 150030, China., Zhou J; College of Plant Protection, Northeast Agricultural University, Harbin 150030, China., Chen M; College of Plant Protection, Northeast Agricultural University, Harbin 150030, China., Li Z; College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China., Song S; College of Plant Protection, Northeast Agricultural University, Harbin 150030, China.; College of Agriculture, Northeast Agricultural University, Harbin 150030, China. |
| Abstrakt: |
Dahlia ( Dahlia variabilis ) is a widely cultivated ornamental and medicinal plant in China. Recently, dahlia plants with symptoms of leaf mottling and distortion were collected in Hohhot, Inner Mongolia, China. The presence of dahlia common mosaic virus (DCMV), an unassigned species in the genus Caulimovirus , was confirmed by high-throughput sequencing. Three fragments of DCMV Inner Mongolia isolate (DCMV-IN) were PCR-amplified with specific primers, sequenced and assembled into the complete genome sequence with a GenBank accession number of OR494328. The double-stranded circular DNA genome of DCMV-IN consists of 7949 bp and contains six open reading frames (ORFs). Sequence analysis showed that DCMV-IN shared high sequence identities with other DCMV isolates available in the GenBank database. Phylogenetic analysis of DCMV isolates and other representative caulimoviruses based on genome sequence clustered four DCMV isolates to a single branch which was closest to dahlia mosaic virus (DMV). No recombination event was detected among the four DCMV isolates. |
