Rhodamine-Based Cyclic Hydroxamate as Fluorescent pH Probe for Imaging of Lysosomes.

Autor: Kim YJ; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea., Jang M; Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea.; New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Republic of Korea., Roh J; Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea.; KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon 34141, Republic of Korea., Lee YJ; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea., Moon HJ; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea., Byun J; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea., Wi J; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea., Ko SK; Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea.; KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon 34141, Republic of Korea., Tae J; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Oct 11; Vol. 24 (20). Date of Electronic Publication: 2023 Oct 11.
DOI: 10.3390/ijms242015073
Abstrakt: Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent pH probes have been of great interest due to their ON/OFF fluorescence depending on the spirolactam ring's opening/closure. By introducing the N -alkyl-hydroxamic acid instead of the alkyl amines in the spirolactam of rhodamine, we were able to tune the pH range where the ring opening and closing of the spirolactam occurs. This six-membered cyclic hydroxamate spirolactam ring of rhodamine B proved to be highly fluorescent in acidic pH environments. In addition, we could monitor pH changes of lysosomes in live cells and zebrafish.
Databáze: MEDLINE
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