| Autor: |
Khedhri S; Laboratory of Management and Valorization of Forest Resources, National Institute of Researches on Rural Engineering, Water and Forests, Ariana 2080, Tunisia., Polito F; Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy., Caputo L; Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy., De Feo V; Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.; Institute of Food Science, National Research Council, Via Roma, 60, 83100 Avellino, Italy., Khamassi M; Laboratory of Management and Valorization of Forest Resources, National Institute of Researches on Rural Engineering, Water and Forests, Ariana 2080, Tunisia., Kochti O; Laboratory of Biotechnology and Nuclear Technology, National Center of Nuclear Science and Technology, Sidi Thabet, Ariana 2020, Tunisia., Hamrouni L; Laboratory of Management and Valorization of Forest Resources, National Institute of Researches on Rural Engineering, Water and Forests, Ariana 2080, Tunisia., Mabrouk Y; Laboratory of Biotechnology and Nuclear Technology, National Center of Nuclear Science and Technology, Sidi Thabet, Ariana 2020, Tunisia., Nazzaro F; Institute of Food Science, National Research Council, Via Roma, 60, 83100 Avellino, Italy., Fratianni F; Institute of Food Science, National Research Council, Via Roma, 60, 83100 Avellino, Italy., Amri I; Laboratory of Biotechnology and Nuclear Technology, National Center of Nuclear Science and Technology, Sidi Thabet, Ariana 2020, Tunisia. |
| Abstrakt: |
This study was conducted to examine the chemical composition of the essential oils (EOs) from six Tunisian Eucalyptus species and to evaluate their anti-enzymatic and antibiofilm activities. The EOs were obtained through hydro-distillation of dried leaves and subsequently analyzed using GC/MS. The main class of compounds was constituted by oxygenated monoterpenes, particularly prominent in E. brevifolia (75.7%), E. lehmannii (72.8%), and E. woollsiana (67%). Anti-enzymatic activities against cholinesterases, α-amylase, and α-glucosidase were evaluated using spectrophotometric methods. Notably, the E. brevifolia , E. extensa , E. leptophylla , E. patellaris , and E. woollsiana EOs displayed potent acetylcholinesterase (AChE) inhibition (IC 50 : 0.25-0.60 mg/mL), with E. lehmannii exhibiting lower activity (IC 50 : 1.2 mg/mL). E. leptophylla and E. brevifolia showed remarkable α-amylase inhibition (IC 50 : 0.88 mg/mL), while E. brevifolia and E. leptophylla significantly hindered α-glucosidase (IC 50 < 30 mg/mL), distinguishing them from other EOs with limited effects. Additionally, the EOs were assessed for their anti-biofilm properties of Gram-positive ( Staphylococcus aureus and Listeria monocytogenes ) and Gram-negative ( Acinetobacter baumannii , Pseudomonas aeruginosa and Escherichia coli ) bacterial strains. The E. extensa EO demonstrated the main antibiofilm effect against E. coli and L. monocytogenes with an inhibition > 80% at 10 mg/mL. These findings could represent a basis for possible further use of Eucalyptus EOs in the treatment of human microbial infections and/or as a coadjutant in preventing and treating Alzheimer's disease and/or diabetes mellitus. |
