| Autor: |
Donoso-Piñol P; Doctoral Program in Science of Natural Resources, University of La Frontera, Temuco 4780000, Chile., Briceño G; Department of Chemistry Science and Natural Resources, University of La Frontera, Temuco 4780000, Chile.; Biotechnological Research Centre Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Temuco 4780000, Chile., Evaristo JAM; Laboratory of Proteomics, LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 22775-000, Brazil., Nogueira FCS; Laboratory of Proteomics, LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 22775-000, Brazil., Leiva B; Doctoral Program in Science of Natural Resources, University of La Frontera, Temuco 4780000, Chile.; Biotechnological Research Centre Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Temuco 4780000, Chile., Lamilla C; Biotechnological Research Centre Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Temuco 4780000, Chile., Schalchli H; Biotechnological Research Centre Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Temuco 4780000, Chile., Diez MC; Biotechnological Research Centre Applied to the Environment (CIBAMA-BIOREN), University of La Frontera, Temuco 4780000, Chile.; Department of Chemical Engineering, University of La Frontera, Temuco 4780000, Chile. |
| Abstrakt: |
The fungicide iprodione (IPR) (3-(3,5-dichlorophenyl) N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide) is a highly toxic compound. Although IPR has been restricted, it is still being applied in many places around the world, constituting an environmental risk. The biodegradation of IPR is an attractive option for reducing its residues. In this study, we isolated thirteen IPR-tolerant bacteria from a biopurification system designed to treat pesticides. A study of biodegradation using different strains was comparatively evaluated, and the best degradation rate of IPR was presented by Achromobacter sp. C1 with a half-life (T 1/2 ) of 9 days. Based on a nano-LC-MS/MS analysis for the strains, proteins solely expressed in the IPR treatment were identified by highlighting the strain Achromobacter sp. C1, with 445 proteins primarily involved in the biosynthesis of secondary metabolites and microbial metabolism in diverse environments. Differentially expressed protein amidases were involved in six metabolic pathways. Interestingly, formamidase was inhibited while other cyclases, i.e., amidase and mandelamide hydrolase, were overexpressed, thereby minimizing the effect of IPR on the metabolism of strain C1. The dynamic changes in the protein profiles of bacteria that degrade IPR have been poorly studied; therefore, our results offer new insight into the metabolism of IPR-degrading microorganisms, with special attention paid to amidases. |
