Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease.

Autor: Søgaard SB; Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.; Department of Diagnostic Radiology, Rigshospitalet, 2100 Copenhagen, Denmark., Andersen SB; Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.; Department of Diagnostic Radiology, Rigshospitalet, 2100 Copenhagen, Denmark., Taghavi I; Center for Fast Ultrasound Imaging, Department of Health Technology, Technical University of Denmark, 2800 Lyngby, Denmark., Schou M; BK Medical, 2730 Herlev, Denmark., Christoffersen C; Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.; Department of Clinical Biochemistry, Rigshospitalet, 2100 Copenhagen, Denmark., Jacobsen JCB; Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark., Kjer HM; Department of Applied Mathematics and Computer Science, Technical University of Denmark, 2800 Lyngby, Denmark., Gundlach C; Department of Physics, Technical University of Denmark, 2800 Lyngby, Denmark., McDermott A; Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark., Jensen JA; Center for Fast Ultrasound Imaging, Department of Health Technology, Technical University of Denmark, 2800 Lyngby, Denmark., Nielsen MB; Department of Diagnostic Radiology, Rigshospitalet, 2100 Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, 2200 Copenhagen, Denmark., Sørensen CM; Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2023 Oct 12; Vol. 13 (20). Date of Electronic Publication: 2023 Oct 12.
DOI: 10.3390/diagnostics13203197
Abstrakt: Individuals with diabetes at risk of developing diabetic kidney disease (DKD) are challenging to identify using currently available clinical methods. Prognostic accuracy and initiation of treatment could be improved by a quantification of the renal microvascular rarefaction and the increased vascular tortuosity during the development of DKD. Super-resolution ultrasound (SRUS) imaging is an in vivo technique capable of visualizing blood vessels at sizes below 75 µm. This preclinical study aimed to investigate the alterations in renal blood vessels' density and tortuosity in a type 2 diabetes rat model, Zucker diabetic fatty (ZDF) rats, as a prediction of DKD. Lean age-matched Zucker rats were used as controls. A total of 36 rats were studied, subdivided into ages of 12, 22, and 40 weeks. Measured albuminuria indicated the early stage of DKD, and the SRUS was compared with the ex vivo micro-computed tomography (µCT) of the same kidneys. Assessed using the SRUS imaging, a significantly decreased cortical vascular density was detected in the ZDF rats from 22 weeks of age compared to the healthy controls, concomitant with a significantly increased albuminuria. Already by week 12, a trend towards a decreased cortical vascular density was found prior to the increased albuminuria. The quantified vascular density in µCT corresponded with the in vivo SRUS imaging, presenting a consistently lower vascular density in the ZDF rats. Regarding vessel tortuosity, an overall trend towards an increased tortuosity was present in the ZDF rats. SRUS shows promise for becoming an additional tool for monitoring and prognosing DKD. In the future, large-scale animal studies and human trials are needed for confirmation.
Competing Interests: M.S. is an employee at BK Medical ApS, Herlev, Denmark.
Databáze: MEDLINE
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