MiR-483-5p downregulation alleviates ox-LDL induced endothelial cell injury in atherosclerosis.

Autor: Zhu H; Department of Geriatrics, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, China., Liang H; Department of Geriatrics, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, China., Gao Z; Department of Cardiology, Taihe Hospital, Hubei University of Medicine, No. 32 Renminnan Road, Shiyan, 442000, China., Zhang X; Department of Geriatrics, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, China., He Q; Department of Cardiology, Taihe Hospital, Hubei University of Medicine, No. 32 Renminnan Road, Shiyan, 442000, China., He C; Department of Cardiology, Taihe Hospital, Hubei University of Medicine, No. 32 Renminnan Road, Shiyan, 442000, China., Cai C; Department of Cardiology, Taihe Hospital, Hubei University of Medicine, No. 32 Renminnan Road, Shiyan, 442000, China. chao610zong@163.com., Chen J; Department of Cardiology, Taihe Hospital, Hubei University of Medicine, No. 32 Renminnan Road, Shiyan, 442000, China. chenjiajuan0719@163.com.
Jazyk: angličtina
Zdroj: BMC cardiovascular disorders [BMC Cardiovasc Disord] 2023 Oct 27; Vol. 23 (1), pp. 521. Date of Electronic Publication: 2023 Oct 27.
DOI: 10.1186/s12872-023-03496-1
Abstrakt: Background: In light of the abnormal expression of microRNA (miR-483-5p) in patients with atherosclerosis (AS), its role in vascular endothelial cell injury was explored. And the mechanisms related to autophagy were also elucidated.
Methods: Human umbilical vein endothelial cells (HUVECs) were given 100 mg/L ox-LDL to induce endothelial injury. Cell transfection was done to regulate miR-483-5p levels. Cell viability and apoptosis were detected. qRT-PCR was employed for the mRNA levels' detection.
Results: Autophagic flux impairment of HUVECs was detected after ox-LDL treatment, along with the upregulation of miR-483-5p. Ox-LDL inhibited cell viability and promoted cell apoptosis, but these influences were changed by miR-483-5p downregulation. MiR-483-5p downregulation decreased the mRNA levels of IL-1β, IL-6, ICAM-1 and VCAM-1. 3-MA, the autophagy inhibitor, reversed the beneficial role of miR-483-5p downregulation in ox-LDL-induced HUVECs' injury. TIMP2 acts as a target gene of miR-483-5p, and was downregulated in HUVEC models.
Conclusion: MiR-483-5p downregulation alleviated ox-LDL-induced endothelial injury via activating autophagy, this might be related to TIMP2.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: