Total infectome investigation of diphtheritic stomatitis in yellow-eyed penguins (Megadyptes antipodes) reveals a novel and abundant megrivirus.

Autor: Wierenga JR; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand; Wildbase, School of Veterinary Science, Massey University, New Zealand., Grimwood RM; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Taylor HS; Biodiversity Group, Department of Conservation/Te Papa Atawhai, New Zealand; Diagnostic and Surveillance Services, Biosecurity New Zealand, Ministry for Primary Industries, New Zealand., Hunter S; Wildbase, School of Veterinary Science, Massey University, New Zealand., Argilla LS; Wildlife Hospital, Dunedin, Otago Polytechnic School of Veterinary Nursing, New Zealand., Webster T; Yellow-eyed Penguin Trust, New Zealand., Lim L; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., French R; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Schultz H; Biodiversity Group, Department of Conservation/Te Papa Atawhai, New Zealand., Jorge F; Otago Micro and Nano Imaging, University of Otago, Dunedin, New Zealand., Bostina M; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Burga L; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Swindells-Wallace P; Hoiho Technical Group, Te Rūnanga o Ngāi Tahu, New Zealand., Holmes EC; Sydney Institute for Infectious Diseases, School of Medical Sciences, The University of Sydney, Australia., McInnes K; Biodiversity Group, Department of Conservation/Te Papa Atawhai, New Zealand., Morgan KJ; Wildbase, School of Veterinary Science, Massey University, New Zealand., Geoghegan JL; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand; Institute of Environmental Science and Research, Wellington, New Zealand. Electronic address: jemma.geoghegan@otago.ac.nz.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2023 Nov; Vol. 286, pp. 109895. Date of Electronic Publication: 2023 Oct 20.
DOI: 10.1016/j.vetmic.2023.109895
Abstrakt: First identified in 2002, diphtheritic stomatitis (DS) is a devastating disease affecting yellow-eyed penguins (Megadyptes antipodes, or hoiho in te reo Māori). The disease is associated with oral lesions in chicks and has caused significant morbidity and mortality. DS is widespread among yellow-eyed penguin chicks on mainland New Zealand yet appears to be absent from the subantarctic population. Corynebacterium spp. have previously been suspected as causative agents yet, due to inconsistent cultures and inconclusive pathogenicity, their role in DS is unclear. Herein, we used a metatranscriptomic approach to identify potential causative agents of DS by revealing the presence and abundance of all viruses, bacteria, fungi and protozoa - together, the infectome. Oral and cloacal swab samples were collected from presymptomatic, symptomatic and recovered chicks along with a control group of healthy adults. Two novel viruses from the Picornaviridae were identified, one of which - yellow-eyed penguin megrivirus - was highly abundant in chicks irrespective of health status but not detected in healthy adults. Tissue from biopsied oral lesions also tested positive for the novel megrivirus upon PCR. We found no overall clustering among bacteria, protozoa and fungi communities at the genus level across samples, although Paraclostridium bifermentans was significantly more abundant in oral microbiota of symptomatic chicks compared to other groups. The detection of a novel and highly abundant megrivirus has sparked a new line of inquiry to investigate its potential association with DS.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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