| Autor: |
Ju WS; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Seo K; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Lee BR; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Park MR; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Lee MG; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Byun SJ; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Yang H; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Kim Y; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea., Ock SA; Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of Korea. |
| Abstrakt: |
This study examined the potential benefits of male specific-pathogen-free (SPF) White Leghorn embryos in cellular agriculture for sustainable and ethical poultry meat production-addressing traditional farming challenges, including disease outbreaks of Salmonella and Avian influenza. We isolated myogenic precursor cells (MPCs) from the thigh muscles ( Musculus femoris ) of 12.5-day-old embryos from 10 SPF White Leghorns that tested negative for Salmonella . We randomly selected MPCs from three males and three females, isolated them using a modified pre-plating (pp) method, and compared their in vitro development. After 1 h (pp1) and 2 h (pp2) of incubation, they were transferred to a new dish to remove fast-adhering cells and cultured (pp3). Isolated MPCs had a 69% positive reaction to Pax7. During proliferation, no differences were observed in PAX7, MYF5 , or MYOD expression between the male and female MPCs. However, after five days of differentiation, the expression of late myogenic factors- MYOG and MYF6- significantly increased in all MPCs. Notably, MYOG expression was 1.9 times higher in female than in male MPCs. This impacted MYMK 's expression pattern. Despite this, the myotube fusion index did not differ between the sexes. Muscle cells from male SPF-laying chicken embryos are promising for developing clean animal-cell-derived protein sources via resource recycling. |
