| Autor: |
Ponomarev VA; National University of Science and Technology 'MISIS', Moscow 119049, Russia., Sheveyko AN; National University of Science and Technology 'MISIS', Moscow 119049, Russia., Kuptsov KA; National University of Science and Technology 'MISIS', Moscow 119049, Russia., Sukhanova EV; Emanuel Institute of Biochemical Physics RAS, Moscow 199339, Russia., Popov ZI; Emanuel Institute of Biochemical Physics RAS, Moscow 199339, Russia.; Plekhanov Russian University of Economics, 36 Stremyanny per., Moscow 117997, Russia., Permyakova ES; National University of Science and Technology 'MISIS', Moscow 119049, Russia., Slukin PV; State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Russia., Ignatov SG; State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Russia., Ilnitskaya AS; N.N. Blokhin National Medical Research Center of Oncology, Kashirskoe Shosse 24, Moscow 115478, Russia., Gloushankova NA; N.N. Blokhin National Medical Research Center of Oncology, Kashirskoe Shosse 24, Moscow 115478, Russia., Timoshenko RV; National University of Science and Technology 'MISIS', Moscow 119049, Russia., Erofeev AS; National University of Science and Technology 'MISIS', Moscow 119049, Russia., Kuchmizhak AA; Institute for Automation and Control Processes, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia.; Pacific Quantum Center, Far Eastern Federal University, Vladivostok 690922, Russia., Shtansky DV; National University of Science and Technology 'MISIS', Moscow 119049, Russia. |
| Abstrakt: |
Bone implants with biocompatibility and the ability to biomineralize and suppress infection are in high demand. The occurrence of early infections after implant placement often leads to repeated surgical treatment due to the ineffectiveness of antibiotic therapy. Therefore, an extremely attractive solution to this problem would be the ability to initiate bacterial protection of the implant by an external influence. Here, we present a proof-of-concept study based on the generation of reactive oxygen species (ROS) by the implant surface in response to X-ray irradiation, including through a layer of 3 mm adipose tissue, providing bactericidal protection. The effect of UV and X-ray irradiation of the implant surface on the ROS formation and the associated bactericidal activity was compared. The focus of our study was light-sensitive Si-doped TiCaCON films decorated with Fe and Pt nanoparticles (NPs) with photoinduced antibacterial activity mediated by ROS. In the visible and infrared range of 300-1600 nm, the films absorb more than 60% of the incident light. The high light absorption capacity of TiO 2 /TiC and TiO 2 /TiN heterostructures was demonstrated by density functional theory calculations. After short-term (5-10 s) low-dose X-ray irradiation, the films generated significantly more ROS than after UV illumination for 1 h. The Fe/TiCaCON-Si films showed enhanced biomineralization capacity, superior cytocompatibility, and excellent antibacterial activity against multidrug-resistant hospital Escherichia coli U20 and K261 strains and methicillin-resistant Staphylococcus aureus MW2 strain. Our study clearly demonstrates that oxidized Fe NPs are a promising alternative to the widely used Ag NPs in antibacterial coatings, and X-rays can potentially be used in ROS-regulating therapy to suppress inflammation in case of postimplant complications. |
