| Autor: |
Lokhov PG; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia., Balashova EE; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia., Maslov DL; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia., Trifonova OP; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia., Lisitsa AV; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia., Markova YM; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Stetsenko VV; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Polyanina AS; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Sheveleva SA; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Sharafetdinov KK; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Nikityuk DB; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Tutelyan VA; Federal State Budgetary Institution of Science, Federal Research Centre of Nutrition, Biotechnology and Food Safety, Ustinsky Passage 2/14, 109240 Moscow, Russia., Archakov AI; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia. |
| Abstrakt: |
Recently, a clinical blood metabogram was developed as a fast, low-cost and reproducible test that allows the implementation of metabolomics in clinical practice. The components of the metabogram are functionally related groups of blood metabolites associated with humoral regulation, the metabolism of lipids, carbohydrates and amines, lipid intake into the organism, and liver function, thereby providing clinically relevant information. It is known that the gut microbiota affects the blood metabolome, and the components of the blood metabolome may affect the composition of the gut microbiota. Therefore, before using the metabogram in the clinic, the link between the metabogram components and the level of gut microorganisms should be established. For this purpose, the metabogram and microbiota data were obtained in this work for the same individuals. Metabograms of blood plasma were obtained by direct mass spectrometry of blood plasma, and the gut microbiome was determined by a culture-based method and real-time polymerase chain reaction (PCR). This study involved healthy volunteers and individuals with varying degrees of deviation in body weight ( n = 44). A correlation analysis determined which metabogram components are linked to which gut microorganisms and the strength of this link. Moreover, diagnostic parameters (sensitivity, specificity and accuracy) confirmed the capacity of metabogram components to be used for diagnosing gut microbiota alterations. Therefore, the obtained results allow the use of the metabogram in a clinical setting, taking into account its relationship with gut microbiota. |
