| Autor: |
Mavroidi A; Department of Microbiology, General University Hospital of Patras, 26504 Patras, Greece., Gartzonika K; Department of Microbiology, Medical School, Ioannina University Hospital, 45110 Ioannina, Greece., Spanakis N; Department of Microbiology, Medical School, University of Athens, 11527 Athens, Greece., Froukala E; Department of Microbiology, Medical School, University of Athens, 11527 Athens, Greece., Kittas C; Department of Microbiology, Medical School, Ioannina University Hospital, 45110 Ioannina, Greece., Vrioni G; Department of Microbiology, Medical School, University of Athens, 11527 Athens, Greece., Tsakris A; Department of Microbiology, Medical School, University of Athens, 11527 Athens, Greece. |
| Jazyk: |
angličtina |
| Zdroj: |
Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2023 Oct 18; Vol. 12 (10). Date of Electronic Publication: 2023 Oct 18. |
| DOI: |
10.3390/antibiotics12101549 |
| Abstrakt: |
Nosocomial outbreaks of multidrug-resistant (MDR) Enterobacter cloacae complex (ECC) are often reported worldwide, mostly associated with a small number of multilocus-sequence types of E. hormaechei and E. cloacae strains. In Europe, the largest clonal outbreak of bla NDM-1 -producing ECC has been recently reported, involving an ST182 E. hormaechei strain in a Greek teaching hospital. In the current study, we aimed to further investigate the genetic make-up of two representative outbreak isolates. Comparative genomics of whole genome sequences (WGS) was performed, including whole genome-based taxonomic analysis and in silico prediction of virulence determinants of the bacterial cell surface, plasmids, antibiotic resistance genes and virulence factors present on genomic islands. The enterobacterial common antigen and the colanic antigen of the cell surface were identified in both isolates, being similar to the gene clusters of the E. hormaechei ATCC 49162 and E. cloacae ATCC 13047 type strains, whereas the two strains possessed different gene clusters encoding lipopolysaccharide O-antigens. Other virulence factors of the bacterial cell surface, such as flagella, fimbriae and pili, were also predicted to be encoded by gene clusters similar to those found in Enterobacter spp. and other Enterobacterales. Secretion systems and toxin-antitoxin systems, which also contribute to pathogenicity, were identified. Both isolates harboured resistance genes to multiple antimicrobial classes, including β-lactams, aminoglycosides, quinolones, chloramphenicol, trimethoprim, sulfonamides and fosfomycin; they carried bla TEM-1 , bla OXA-1 , bla NDM-1 , and one of them also carried bla CTXM-14 , bla CTXM-15 and bla LAP-2 plasmidic alleles. Our comprehensive analysis of the WGS assemblies revealed that bla NDM-1 -producing outbreak isolates possess components of the bacterial cell surface as well as genomic islands, harbouring resistance genes to several antimicrobial classes and various virulence factors. Differences in the plasmids carrying β-lactamase genes between the two strains have also shown diverse modes of acquisition and an ongoing evolution of these mobile elements. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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