Therapeutic efficacy of β-sitosterol treatment on Trypanosoma congolense infection, anemia development, and trans-sialidase ( TconTS1 ) gene expression.
| Autor: | Aminu S; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.; Chemical and Biochemical Sciences-Green Processing Engineering, Mohammed VI Polytechnic University, Ben Guerir, Morocco., Chechet GD; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.; African Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria., Alkhalil SS; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Alquwayiyah, Saudi Arabia., Sobeh M; AgroBioSciences Program, College for Sustainable Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben Guerir, Morocco., Daoud R; Chemical and Biochemical Sciences-Green Processing Engineering, Mohammed VI Polytechnic University, Ben Guerir, Morocco., Simelane MB; Department of Biochemistry, University of Johannesburgs, Johannesburg, South Africa., Onyike E; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria., Ibrahim MA; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.; African Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2023 Sep 27; Vol. 14, pp. 1282257. Date of Electronic Publication: 2023 Sep 27 (Print Publication: 2023). |
| DOI: | 10.3389/fmicb.2023.1282257 |
| Abstrakt: | Background: African animal trypanosomiasis hinders sustainable livestock productivity in sub-Saharan Africa. About 17 million infected cattle are treated with trypanocides annually but most of the drugs are associated with drawbacks, necessitating the search for a promising chemotherapeutic agent. Objectives: In this study, the effects of β-sitosterol on Trypanosoma congolense infection were investigated along with its effect on the trans-sialidase gene expressions. Results: Oral treatment with β-sitosterol at 15 and 30 mg/kg body weight (BW) for 14 days significantly ( p < 0.05) reduced parasitemia and ameliorated the parasite-induced anemia. Also, the parasite-induced increase in serum urea level and renal histopathological damage scores in addition to renal hypertrophy was significantly ( p < 0.05) reverted following treatment with 30 mg/kg BW β-sitosterol. The compound also significantly ( p < 0.05) down-regulated the expression of TconTS1 but not TconTS2 , TconTS3 , and TconTS4 . Correlation analysis between free serum sialic acid with the TconTS1 and TconTS2 gene variants revealed negative correlations in the β-sitosterol-treated groups although they were non-significant ( p > 0.05) in the group treated with 15 mg/kg BW β-sitosterol. Similarly, a non-significant negative ( p > 0.05) correlation between the biomolecule and the TconTS3 and TconTS4 gene variants was observed in the β-sitosterol-treated groups while positive correlations were observed in the infected untreated control group. Conclusion: The observed effect of β-sitosterol on T. congolense infection could make the compound a possible template for the design of novel trypanocides. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2023 Aminu, Chechet, Alkhalil, Sobeh, Daoud, Simelane, Onyike and Ibrahim.) |
| Databáze: | MEDLINE |
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