Cellular traits regulate fluorescence-based light-response phenotypes of coral photosymbionts living in-hospite .

Autor: McQuagge A; Department of Biology, University of Alabama, Tuscaloosa, AL, United States.; Dauphin Island Sea Lab, Dauphin Island, AL, United States., Pahl KB; Department of Biology, University of Alabama, Tuscaloosa, AL, United States.; Dauphin Island Sea Lab, Dauphin Island, AL, United States., Wong S; Dauphin Island Sea Lab, Dauphin Island, AL, United States.; Department of Environmental Science, University of Virginia, Charlottesville, VA, United States., Melman T; Reef Systems Coral Farm, New Albany, OH, United States., Linn L; Dauphin Island Sea Lab, Dauphin Island, AL, United States., Lowry S; Department of Biology, University of Alabama, Tuscaloosa, AL, United States.; Dauphin Island Sea Lab, Dauphin Island, AL, United States., Hoadley KD; Department of Biology, University of Alabama, Tuscaloosa, AL, United States.; Dauphin Island Sea Lab, Dauphin Island, AL, United States.
Jazyk: angličtina
Zdroj: Frontiers in physiology [Front Physiol] 2023 Oct 11; Vol. 14, pp. 1244060. Date of Electronic Publication: 2023 Oct 11 (Print Publication: 2023).
DOI: 10.3389/fphys.2023.1244060
Abstrakt: Diversity across algal family Symbiodiniaceae contributes to the environmental resilience of certain coral species. Chlorophyll- a fluorescence measurements are frequently used to determine symbiont health and resilience, but more work is needed to refine these tools and establish how they relate to underlying cellular traits. We examined trait diversity in symbionts from the generas Cladocopium and Durusdinium, collected from 12 aquacultured coral species. Photophysiological metrics (Φ PSII , σ PSII , ρ, τ 1 , τ 2 , antenna bed quenching, non-photochemical quenching, and qP) were assessed using a prototype multi-spectral fluorometer over a variable light protocol which yielded a total of 1,360 individual metrics. Photophysiological metrics were then used to establish four unique light-response phenotypic variants. Corals harboring C15 were predominantly found within a single light-response phenotype which clustered separately from all other coral fragments. The majority of Durusdinium dominated colonies also formed a separate light-response phenotype which it shared with a few C1 dominated corals. C15 and D1 symbionts appear to differ in which mechanisms they use to dissipate excess light energy. Spectrally dependent variability is also observed across light-response phenotypes that may relate to differences in photopigment utilization. Symbiont cell biochemical and structural traits (atomic C:N:P, cell size, chlorophyll- a , neutral lipid content) was also assessed within each sample and differ across light-response phenotypes, linking photophysiological metrics with underlying primary cellular traits. Strong correlations between first- and second-order traits, such as Quantum Yield and cellular N:P content, or light dissipation pathways (qP and NPQ) and C:P underline differences across symbiont types and may also provide a means for using fluorescence-based metrics as biomarkers for certain primary-cellular traits.
Competing Interests: Author TM was employed by Reef Systems Coral Farm. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 McQuagge, Pahl, Wong, Melman, Linn, Lowry and Hoadley.)
Databáze: MEDLINE
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