Before and After: Evaluation of Microbial and Organic Loads in Produce Handling and Packing Operations with Diverse Cleaning and Sanitizing Procedures.
| Autor: | Ohman E; Food Innovation Center, Oregon State University, 1207 NW Naito Parkway, Portland, OR 97209, USA., Kilgore S; Wiegand Hall, Oregon State University, 3051 SW Campus Way, Corvallis, OR 97331, USA., Waite-Cusic J; Wiegand Hall, Oregon State University, 3051 SW Campus Way, Corvallis, OR 97331, USA., Kovacevic J; Food Innovation Center, Oregon State University, 1207 NW Naito Parkway, Portland, OR 97209, USA. Electronic address: jovana.kovacevic@oregonstate.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of food protection [J Food Prot] 2023 Dec; Vol. 86 (12), pp. 100185. Date of Electronic Publication: 2023 Oct 24. |
| DOI: | 10.1016/j.jfp.2023.100185 |
| Abstrakt: | Inadequate cleaning and/or sanitation (C/S) of food contact surfaces (FCSs) has been frequently reported during Produce Safety Rule inspections; however, limited data are available evaluating the effectiveness of C/S processes in produce operations. Different C/S practices were evaluated in four fresh produce operations for their efficacy in reducing microbial and organic loads on various FCSs. Microbial (aerobic plate counts; APC) and organic (ATP) loads were quantified during production, after cleaning, and after sanitizing, if applicable. Operations included: a berry packinghouse (BerryPK; wet cleaning), a blueberry harvest contractor (BerryHC; cleaning + sanitizing, C+S), and two mixed vegetable packinghouses (MixedV1; C+S, and MixedV2; rinsing + sanitizing, R+S). Following wet cleaning, significant reductions in APCs (p < 0.05) were seen on high-density polyethylene (HDPE) storage trays (n = 50) in BerryPK (3.1 ± 0.9 to 2.5 ± 0.7 log CFU/100 cm 2 ). In BerryHC, a greater reduction in APCs was seen on HDPE harvest buckets (n = 25) following C+S (3.8 ± 0.5 to 1.1 ± 0.4 log CFU/100 cm 2 ), compared to wet cleaning only in BerryPK. Stainless steel and conveyor belt FCSs (n = 16) in MixedV1 were sampled, and a significant reduction in APCs (p < 0.05) was observed when comparing in-use (4.8 ± 1.3 log CFU/100 cm 2 ) to post-C+S (3.9 ± 0.7 log CFU/100 cm 2 ). When similar FCSs (n = 17) were sampled in MixedV2, R+S also led to significant reduction in APCs (3.3 ± 0.6 to 1.9 ± 0.6 log CFU/100 cm 2 ) (p < 0.05). ATP testing in fresh produce settings yielded inconsistent results, with no correlation between organic and bacterial loads detected during production (R 2 = 0.00) across four operations, and weak correlations observed after cleaning (R 2 = 0.18) and after sanitation (R 2 = 0.33). The results from this study provide the foundational basis for future research on practical and effective C/S methods tailored to the produce industry. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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