A marker-free genome editing method in S. pombe using the delitto perfetto approach.

Autor:	Fréon K; Institut Curie, PSL Research University, UMR3348, 91400 Orsay, France.; Paris-Saclay University, 91400 Orsay, France.; CNRS, UMR3348, 91400 Orsay France. Equipe Labellisée ligue contre le Cancer., Lambert SAE; Institut Curie, PSL Research University, UMR3348, 91400 Orsay, France.; Paris-Saclay University, 91400 Orsay, France.; CNRS, UMR3348, 91400 Orsay France. Equipe Labellisée ligue contre le Cancer., Lobachev KS; School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, United States., Ait Saada A; Institut Curie, PSL Research University, UMR3348, 91400 Orsay, France.; Paris-Saclay University, 91400 Orsay, France.; CNRS, UMR3348, 91400 Orsay France. Equipe Labellisée ligue contre le Cancer.
Jazyk:	angličtina
Zdroj:	MicroPublication biology [MicroPubl Biol] 2023 Oct 09; Vol. 2023. Date of Electronic Publication: 2023 Oct 09 (Print Publication: 2023).
DOI:	10.17912/micropub.biology.000997
Abstrakt:	The fission yeast, like budding yeast, offer an easy manipulation of their genome, despite their distinct biology. Most tools available in budding yeast are also available in fission yeast in versions taking into account the features of each organism. The delitto perfetto is a powerful approach, initially developed in S. cerevisiae , for in vivo site-directed mutagenesis. Here, we present an adaptation of the approach to S. pombe manipulation and demonstrate its applicability for a rapid, marker-free and efficient in vivo site-directed mutagenesis and N-terminal tagging of nonessential genes in fission yeast. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2023 by the authors.)
Databáze:	MEDLINE
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