Aeromonashydrophila infection in tilapia triggers changes in the microbiota composition of fish internal organs.

Autor:	Ofek T; Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, 199 Abba Khoushi Ave. Mt. Carmel, Haifa 3498838, Israel.; Central Fish Health Laboratory, Fishery and Aquaculture Department, Ministry of Agriculture and Rural Development, 1 Havazelet St. Nir David 1080300, Israel., Izhaki I; Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, 199 Abba Khoushi Ave. Mt. Carmel, Haifa 3498838, Israel., Halpern M; Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, 199 Abba Khoushi Ave. Mt. Carmel, Haifa 3498838, Israel.; Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa, Oranim, Derech Kiryat Amal, Tivon 3600600, Israel.
Jazyk:	angličtina
Zdroj:	FEMS microbiology ecology [FEMS Microbiol Ecol] 2023 Nov 13; Vol. 99 (12).
DOI:	10.1093/femsec/fiad137
Abstrakt:	Aeromonas hydrophila is a major pathogenic species that causes mass mortality in various freshwater fish species including hybrid tilapia, the main fish species in Israeli aquaculture. Our hypothesis was that A. hydrophila infection may cause changes in the microbiota composition of fish internal organs, and therefore we aimed to study the effect of A. hydrophila infection by injection or by net handling on the microbiota compositions of fish intestine, spleen, and liver. Significant differences in the microbiota composition were found between the internal organs of the diseased and the healthy fish in both experimental setups. Fusobacteriota was the most dominant phylum in the microbiota of healthy fish (∼70%, liver). Cetobacterium was the most abundant genus and relatively more abundant in healthy, compared to diseased fish. When A. hydrophila was inoculated by injection, it was the only pathogenic genus in the spleen and liver of the diseased fish. However, in the handling experiment, Vibrio was also detected in the diseased fish, demonstrating coinfection interactions. Based on these experiments, we conclude that indeed, A. hydrophila infection in tilapia causes changes in the microbiota composition of fish internal organs, and that fish net handling may trigger bacterial infection in freshwater aquaculture. (© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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