Proteomics approach reveals urinary markers for early pregnancy diagnosis in buffaloes.

Autor: Codognoto VM; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil., de Souza FF; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil., Cataldi TR; Department of Genetic, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, Brazil., Labate CA; Department of Genetic, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, Brazil., de Camargo LS; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil., Esteves Trindade PH; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil., da Rosa Filho RR; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Campus São Paulo, São Paulo, Brazil., de Oliveira DJB; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil., Oba E; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Electronic address: eunice.oba@unesp.br.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2024 Jan 06; Vol. 290, pp. 105036. Date of Electronic Publication: 2023 Oct 24.
DOI: 10.1016/j.jprot.2023.105036
Abstrakt: This study aimed to compare urine proteomics from non- and pregnant buffaloes in order to identify potential biomarkers of early pregnancy. Forty-four females underwent hormonal ovulation synchronization and were randomly divided into two experimental groups: inseminated (n = 30) and non-inseminated (n = 14). The pregnant females were further divided into two groups: pregnant at Day 12 (P12; n = 8) and at Day 18 (P18; n = 8) post-ovulation. The non-pregnant group was also subdivided into two groups: non-pregnant at Day 12 (NP12; n = 7) and at Day 18 (NP18; n = 7). Urine was collected from all females on Days 12 or 18. The samples were processed for proteomics. A total of 798 proteins were reported in the urine considering all groups. The differential proteins play essential roles during pregnancy, acting in cellular transport and metabolism, endometrial remodeling, embryonic protection, and degradation of defective proteins. We suggest that some proteins from our study can be considered biomarkers for early pregnancy diagnosis, since they were increased in pregnant buffaloes. SIGNIFICANCE: Macromolecules have been studied for early pregnancy diagnosis, aiming to increase reproductive efficiency in cattle and buffaloes. Direct methods such as rectal palpation and ultrasonography have been considered late. Thus, this study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. The differential proteins found in our study play essential roles during pregnancy, acting in cellular transport and metabolism, endometrial remodeling, embryonic protection, and degradation of defective proteins. We suggest that these proteins can be considered possible biomarkers for early pregnancy diagnosis since they were increased in the pregnant buffaloes.
Competing Interests: Declaration of Competing Interest The authors have declared that no conflict of interest exists.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: