Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined.

Autor: Martin KA; Department of Veterinary Pathology, Iowa State University College of Veterinary Medicine, Ames, Iowa, United States of America., Jesudoss Chelladurai JRJ; Department of Veterinary Pathology, Iowa State University College of Veterinary Medicine, Ames, Iowa, United States of America.; Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America., Bsrat A; Mekelle University College of Veterinary Medicine, Tigray, Ethiopia., Pulaski C; Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America., Lee ACY; Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, United States of America., Starkey LA; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, United States of America., Brewer MT; Department of Veterinary Pathology, Iowa State University College of Veterinary Medicine, Ames, Iowa, United States of America.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2023 Oct 25; Vol. 17 (10), pp. e0011665. Date of Electronic Publication: 2023 Oct 25 (Print Publication: 2023).
DOI: 10.1371/journal.pntd.0011665
Abstrakt: Background: Toxocara canis is a cosmopolitan parasite of dogs that is transmitted transplacentally to puppies resulting in widespread shedding of eggs in the environment. However, it is not clear if there are dominant parasite genotypes that are more common, pathogenic, or likely to be zoonotic.
Methods/principle Findings: Sequences of mitochondrial cox1 gene from adult worms were used to compare parasites from the United States with submitted sequences from parasites isolated from dogs in different countries. Our analysis revealed at least 55 haplotypes. While we expected the North American worms to form a distinct cluster, we found haplotypes of T. canis reported elsewhere existing in this population. Interestingly, combining the sequence data from our study with the available GenBank data, analysis of cox1 sequences results in five distinct clades that are not geographically defined.
Conclusions: The five clades of T. canis revealed in this study potentially have unique life histories, traits, or host preferences. Additional investigation is needed to see if these distinct clades represent cryptic species with clinically useful attributes or genotypes with taxonomic value. Evaluation of common mitochondrial genes may reveal distinct populations of zoonotic T. canis.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Martin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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