Top-Down Proteoform Analysis by 2D MS with Quadrupolar Detection.

Autor: Polák M; Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic.; Faculty of Science, Charles University, Prague 12843, Czech Republic., Palasser M; Center for Chemistry and Biomedicine, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria., Kádek A; Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic., Kavan D; Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic.; Faculty of Science, Charles University, Prague 12843, Czech Republic., Wootton CA; Bruker Daltonics GmbH & Co KG, Fahrenheitstraße 4, 28359 Bremen, Germany., Delsuc MA; Institut de Génétique et de Biologie Moléculaire et Cellulaire, INSERM, U596, CNRS, UMR7104, Université de Strasbourg, 1 rue Laurent Fries, 67404 Illkirch-Graffenstaden, France., Breuker K; Center for Chemistry and Biomedicine, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria., Novák P; Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic.; Faculty of Science, Charles University, Prague 12843, Czech Republic., van Agthoven MA; Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic.; Center for Chemistry and Biomedicine, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2023 Nov 07; Vol. 95 (44), pp. 16123-16130. Date of Electronic Publication: 2023 Oct 25.
DOI: 10.1021/acs.analchem.3c02225
Abstrakt: Two-dimensional mass spectrometry (2D MS) is a multiplexed tandem mass spectrometry method that does not rely on ion isolation to correlate the precursor and fragment ions. On a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS), 2D MS instead uses the modulation of precursor ion radii inside the ICR cell before fragmentation and yields 2D mass spectra that show the fragmentation patterns of all the analytes. In this study, we perform 2D MS for the first time with quadrupolar detection in a dynamically harmonized ICR cell. We discuss the advantages of quadrupolar detection in 2D MS and how we adapted existing data processing techniques for accurate frequency-to-mass conversion. We apply 2D MS with quadrupolar detection to the top-down analysis of covalently labeled ubiquitin with ECD fragmentation, and we develop a workflow for label-free relative quantification of biomolecule isoforms in 2D MS.
Databáze: MEDLINE
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