One-year post-exposure assessment of 14 C-few-layer graphene biodistribution in mice: single versus repeated intratracheal administration.

Autor:	Sallustrau A; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, 91191 Gif-sur-Yvette, France. antoine.sallustrau@cea.fr., Keck M; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SIMos, 91191 Gif-sur-Yvette, France., Barbe P; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SIMos, 91191 Gif-sur-Yvette, France., Georgin D; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, 91191 Gif-sur-Yvette, France. antoine.sallustrau@cea.fr., Fresneau N; Université Paris-Saclay, CEA, CNRS, NIMBE, LICSEN, 91191 Gif-sur-Yvette, France.; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, 91191 Gif-sur-Yvette, France. antoine.sallustrau@cea.fr., Campidelli S; Université Paris-Saclay, CEA, CNRS, NIMBE, LICSEN, 91191 Gif-sur-Yvette, France., Pibaleau B; Université Paris-Saclay, CEA, CNRS, NIMBE, LEDNA, 91191 Gif-sur-Yvette, France., Pinault M; Université Paris-Saclay, CEA, CNRS, NIMBE, LEDNA, 91191 Gif-sur-Yvette, France., Mayne-L'Hermite M; Université Paris-Saclay, CEA, CNRS, NIMBE, LEDNA, 91191 Gif-sur-Yvette, France., Granotier-Beckers C; Université Paris Cité, Inserm, CEA, Stabilité Génétique Cellules Souches et Radiations/iRCM, 92265 Fontenay-aux-Roses, France.; Université Paris-Saclay, Inserm, CEA, Stabilité Génétique Cellules Souches et Radiations/iRCM, 92265 Fontenay-aux-Roses, France., Schlegel M; Université Paris Saclay, CEA, Service de Recherche en Matériaux et Procédés Avancés, 91191 Gif-sur-Yvette, France., González VJ; Instituto Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha, 13071Ciudad Real, Spain., Vázquez E; Instituto Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha, 13071Ciudad Real, Spain., Servent D; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SIMos, 91191 Gif-sur-Yvette, France., Taran F; Université Paris Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, 91191 Gif-sur-Yvette, France. antoine.sallustrau@cea.fr.
Jazyk:	angličtina
Zdroj:	Nanoscale [Nanoscale] 2023 Nov 09; Vol. 15 (43), pp. 17621-17632. Date of Electronic Publication: 2023 Nov 09.
DOI:	10.1039/d3nr03711h
Abstrakt:	Research on graphene-based nanomaterials has experienced exponential growth in the last few decades, driven by their unique properties and their future potential impact on our everyday life. With the increasing production and commercialization of these materials, there is significant interest in understanding their fate in vivo . Herein, we investigated the distribution of 14 C-few-layer graphene ( 14 C-FLG) flakes (lat. dim. ∼ 500 nm) in mice over a period of one year. Furthermore, we compared the effects of repeated low-dose and acute high-dose exposure by tracheal administration. The results showed that most of the radioactivity was found in the lungs in both cases, with longer elimination times in the case of acute high-dose administration. In order to gain deeper insights into the distribution pattern, we conducted ex vivo investigations using μ-autoradiography on tissue sections, revealing the heterogeneous distribution of the material following administration. For the first time, μ-autoradiography was used to conduct a comprehensive investigation into the distribution and potential presence of FLG within lung cells isolated from the exposed lungs. The presence of radioactivity in lung cells strongly suggests internalization of the 14 C-FLG particles. Overall these results show the long-term accumulation of the material in the lungs over one year, regardless of the administration protocol, and the higher biopersistence of FLG in the case of an acute exposure. These findings highlight the importance of the exposure scenario in the context of intratracheal administration, which is of interest in the evaluation of the potential health risks of graphene-based nanomaterials.
Databáze:	MEDLINE
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