MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism.

Autor: Dluzewska J; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland., Dziegielewski W; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland., Szymanska-Lejman M; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland., Gazecka M; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.; Department of Molecular Virology, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland., Henderson IR; Department of Plant Sciences, University of Cambridge, Cambridge, UK., Higgins JD; Department of Genetics and Genome Biology, University of Leicester, Leicester, UK., Ziolkowski PA; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland. pzio@amu.edu.pl.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Oct 23; Vol. 14 (1), pp. 6716. Date of Electronic Publication: 2023 Oct 23.
DOI: 10.1038/s41467-023-42511-z
Abstrakt: Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
Externí odkaz: