Penthorum chinense Prush extract alleviates aflatoxin B 1 -induced toxicity, oxidative stress and apoptosis via mediating Nrf2 signaling pathway in the Bursa of Fabricius of broilers.

Autor: Nabi F; Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China; Department of Poultry Science, Faculty of Veterinary and Animal Science, LUAWMS, Uthal, Pakistan. Electronic address: fazulnabishar@yahoo.com., Tao W; Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China., Li Z; Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China; College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404155, China., Lu Q; Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China; Immunology Research Center of Medical Research Institute, Southwest University, Chongqing, China., Xie J; Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China; Immunology Research Center of Medical Research Institute, Southwest University, Chongqing, China., Sahito B; Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary and Animal Sciences, Ziauddin University, Karachi 75600, Sindh, Pakistan., Buzdar JA; Department of Poultry Science, Faculty of Veterinary and Animal Science, LUAWMS, Uthal, Pakistan., Liu J; Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China; Immunology Research Center of Medical Research Institute, Southwest University, Chongqing, China. Electronic address: dyx4@swu.edu.cn.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2024 Jan; Vol. 275, pp. 109779. Date of Electronic Publication: 2023 Oct 21.
DOI: 10.1016/j.cbpc.2023.109779
Abstrakt: Aflatoxin B1 (AFB 1 ) is the primary mycotoxin that is responsible for the severe issues plaguing poultry farming. The study was aimed to explore the relevant pathways connected with immunity (inflammation, oxidative stress and apoptosis) in an AFB 1 -challenged chicken by using Penthorum chinense Prush extract (PCPE) in Bursa of Fabricius (BF) of broilers. A total one hundred and eighty day-old broilers were divided into six groups: Control, AFB 1 (3 mg/kg feed), Yin-Chen-Hao Tang extract (YCHT) (10 ml/kg feed), and PCPE groups (low 1 g/kg, medium 2 g/kg, and high 3 g/kg PCPE/kg feed) respectively. The results showed that AFB 1 -challenged birds showed significant decrease in growth, BF weight index, serum antioxidant biomarkers and histopathological changes in BF tissues. The mRNA analysis showed that AFB 1 upregulated the apoptosis associated genes (caspase-3, caspase-9, Bak, Bax and p53) and downregulated BCL-2. Additionally, AFB 1 downregulated expression level of Nuclear Factor EF-2 (Nrf2) related genes (Nrf2, HO-1, NQO1 and GCLC) in the BF of broilers. The PCPE treatment showed positive impact on final weight gain, bursal index, and reversing of pathological changes in the BF of AFB 1 -challanged broilers. PCPE ameliorated oxidative stress generated by AFB 1 , as an increase in antioxidant enzyme activities, alleviated histopathological changes in BF, enhanced the Nrf2 expression levels and lowered the apoptosis gene expressions as compared to AFB 1 . The findings revealed that PCPE activated the Nrf2 pathway, antioxidant defense system and modulated the apoptosis in the BF of broiler chicken.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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