Mammalian cells-based platforms for the generation of SARS-CoV-2 virus-like particles.

Autor: Elfayres G; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Paswan RR; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Sika L; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Girard MP; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Khalfi S; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Letanneur C; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada; Department of Biochemistry, Chemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Milette K; Institute of Innovations in Eco-materials, Eco-products and Eco-energies, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Singh A; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada., Kobinger G; University Hospital Research Center and Department of Microbiology and Infectiology, Université Laval, Québec, Canada., Berthoux L; Department of Medical Biology and FRQS SIDA/MI Network, Université du Québec à Trois-Rivières, Trois-Rivières, Canada. Electronic address: lionel.berthoux@uqtr.ca.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2023 Dec; Vol. 322, pp. 114835. Date of Electronic Publication: 2023 Oct 21.
DOI: 10.1016/j.jviromet.2023.114835
Abstrakt: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19. Though many COVID-19 vaccines have been developed, most of them are delivered via intramuscular injection and thus confer relatively weak mucosal immunity against the natural infection. Virus-Like Particles (VLPs) are self-assembled nanostructures composed of key viral structural proteins, that mimic the wild-type virus structure but are non-infectious and non-replicating due to the lack of viral genetic material. In this study, we efficiently generated SARS-CoV-2 VLPs by co-expressing the four SARS-CoV-2 structural proteins, specifically the membrane (M), small envelope (E), spike (S) and nucleocapsid (N) proteins. We show that these proteins are essential and sufficient for the efficient formation and release of SARS-CoV-2 VLPs. Moreover, we used lentiviral vectors to generate human cell lines that stably produce VLPs. Because VLPs can bind to the virus natural receptors, hence leading to entry into cells and viral antigen presentation, this platform could be used to develop novel vaccine candidates that are delivered intranasally.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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