G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival.

Autor: Prakhar P; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India., Bhatt B; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India., Lohia GK; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India., Shah A; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India., Mukherjee T; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India., Kolthur-Seetharam U; Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India., Sundaresan NR; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India., Rajmani RS; Centre for Infectious Disease Research, Indian Institute of Science, Bangalore-, Karnataka, India., Balaji KN; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2023 Oct 23; Vol. 19 (10), pp. e1011731. Date of Electronic Publication: 2023 Oct 23 (Print Publication: 2023).
DOI: 10.1371/journal.ppat.1011731
Abstrakt: Cholesterol derived from the host milieu forms a critical factor for mycobacterial pathogenesis. However, the molecular circuitry co-opted by Mycobacterium tuberculosis (Mtb) to accumulate cholesterol in host cells remains obscure. Here, we report that the coordinated action of WNT-responsive histone modifiers G9a (H3K9 methyltransferase) and SIRT6 (H3K9 deacetylase) orchestrate cholesterol build-up in in vitro and in vivo mouse models of Mtb infection. Mechanistically, G9a, along with SREBP2, drives the expression of cholesterol biosynthesis and uptake genes; while SIRT6 along with G9a represses the genes involved in cholesterol efflux. The accumulated cholesterol in Mtb infected macrophages promotes the expression of antioxidant genes leading to reduced oxidative stress, thereby supporting Mtb survival. In corroboration, loss-of-function of G9a in vitro and pharmacological inhibition in vivo; or utilization of BMDMs derived from Sirt6-/- mice or in vivo infection in haplo-insufficient Sirt6-/+ mice; hampered host cholesterol accumulation and restricted Mtb burden. These findings shed light on the novel roles of G9a and SIRT6 during Mtb infection and highlight the previously unknown contribution of host cholesterol in potentiating anti-oxidative responses for aiding Mtb survival.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Prakhar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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